Human LYPD6B ELISA Kit

Sample

serum, plasma, cell culture supernatant

Species Reactivity

Human

Intended Use

The Human LYPD6B ELISA Kit is used for the quantitative measurement of human LYPD6B in serum, plasma, and cell culture supernatant.
Individual users should determine appropriate conditions when using other types of samples.
This assay kit is for research use only and not for use in diagnostic or therapeutic procedures.

Storage

- Upon receipt store all components at 4°C.
- Don't expose reagents to excessive light.

Precision

Intra-assay (Within-Run, n=16) CV=4.2, 3.8, 4.2 %
Inter-assay (Run-to-Run, n=5) CV=4.3, 6.4, 7.2 %

Sensitivity

The limit of detection (defined as such a concentration of human LYPD6B giving absorbance higher than mean absorbance of blank* plus three standard deviations of the absorbance of blank: A blank + 3SD blank) is better than 9.2 pg/mL of sample.

General Description

LYPD6B (Ly6/PLAUR domain-containing protein 6B), a member of Ly-6 superfamily, is isolated from human testis cDNA library. Ly-6 (lymphocyte antigen 6) superfamily members are cysteine-rich, normally GPI (glycosyl phosphatidylinositol)-anchored cell surface proteins, which have definite or putative immune related roles (3,4). LYPD6B contains signal peptide (N-terminal), UPAR/Ly6 domain, propeptide (C-terminal) and mature human LYPD6B is 125 amino acids in length and especially highly expressed in testis, lung, stomach, and prostate and localized in the cytoplasm. LYPD6B overexpressed in HEK 293T cells is able to activate the transcriptional activities of AP1 (PMA), but a reason of this event is unclear.