Rat Immunoglobulin Isotyping ELISA Kit (DEIA6802V2)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
10x96T
Sample
supernatants, cell cultures
Species Reactivity
Rat
Intended Use
This Kit contains the necessary reagents, controls, buffers and diluents for performing qualititative enzyme-linked immunosorbent assays (ELISA). This ELISA set is specifically engineered for measurement of rat Immunoglobulins from supernatants from cell cultures.
Contents of Kit
Capture Antibody: Pre-titrated, purified anti-rat Ig monoclonal antibodies:
1. 1 mouse anti-rat kappa light chain 250x 100µl
2. 1 mouse anti-rat lambda light chain 250x 100µl
3. 1 mouse anti-rat IgG1 250x 100µl
4. 1 mouse anti-rat IgG2a 250x 100µl
5. 1 mouse anti-rat IgG2b 250x 100µl
6. 1 mouse anti-rat IgG2c 250x 100µl
7. 1 mouse anti-rat IgA 250x 100µl
8. 1 mouse anti-rat IgM 250x 100µl
Detection Antibody: Pre-titrated, HRP-conjugated anti-rat Ig(H+L) polyclonal antibody
1 vial (500 µl) Detection Antibody Concentrate (250x)
Control: Rat Ig isotype control mixture for generating positive controls
10 vials rat Ig Positive Controls (lyophilized)
Coating Buffer: 1 vial (12 ml) Phosphate Buffered Saline Concentrate (PBS) 10x
Assay Buffer A: 1 bottle (50 ml) Assay Buffer A Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution
1 bottle (120 ml)
10 96-well plates
Storage
2-8°C.
General Description
This Rat Immunoglobulin Isotyping ELISA Kit contains the necessary reagents, controls, buffers and diluents for performing qualititative enzyme-linked immunosorbent assays (ELISA). This ELISA set is specifically engineered for measurement of rat Immunoglobulins from supernatants from cell cultures.This CD Kits are the optimal match for screening your hybridoma in a 96-strip well (8x12) ELISA format. Up to 12 individual samples can be tested for anti-IgG1, IgG2a, IgG2b, IgG2c, IgA, and IgM as well as light chain kappa and lambda. To match your individual research needs and budget, choose between the pre-coated, a coat-it-yourself isotyping kit.

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