Leptospira IgM ELISA Kit

Name: Leptospira IgM ELISA Kit
SKU: DEIA05739M
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

Leptospira IgM is quantitative and qualitative test for detection of genus-specific human antibodies against Leptospira in serum or plasma. For sale in the U.S. for Research Use Only. Not for use in diagnostic procedures.

Contents of Kit

1. Break apart microtiter test strips each with 8 antigen coated single wells (altogether 96), 1 frame, the coating material is inactivated. 12
2. Standard serum (ready-to-use), Human serum in phosphate buffer with protein; negative for anti-HIV-Ab, anti-HBs-Ag (Hepatitis B-Virus-surface antigen) and anti-HCV-Ab; preservative: < 0.1 % sodium azide, coloring: Amaranth O. 2 × 2 ml.
3. Negative control serum (ready-to-use), Human serum in phosphate buffer with protein; negative for anti-HIV, anti-HBs (Hepatitis BVirus-surface antigen) and anti-HCV; preservative: < 0.1 % sodium azide coloring: Lissamin green V. 2 ml.
4. Anti-human-IgM-conjugate (ready-to-use), Anti-human-IgM from goat (polyclonal), conjugated to alkaline phosphatase, stabilized with protein stabilization solution preservative: 0.01 % methylisothiazolone, 0.01 % bromnitrodioxane. 13 ml.
5. Washing solution concentrate (sufficient for 1 litre), Sodium chloride solution with Tween 20, 30 mM Tris preservative: < 0.1 % sodium azide. 33.3 ml
6. Dilution buffer, Phosphate buffer with protein and Tween 20; preservative: < 0.1 % sodium azide, 0.01 g/l Bromphenol blue sodium salt. 2 × 50 ml
7. Stopping solution, 1.2 N sodium hydroxide. 15 ml
8. Substrate (ready-to-use), Para-nitrophenylphosphate, solvent free buffer, preservative: < 0.1 % sodium azide. (Substrate in unopened bottle may have a slightly yellow color. This does not reduce the quality of the product!) 13 ml.

Storage

Reagent	Storage	Stability
microtiter strips (antigen)	after opening at 2-8°C in closed aluminum bag with desiccant. Strips which are not used must be stored in the press-seal bag of aluminum compound foil under dry and airtight conditions!	4 weeks
control sera / standard sera	after opening at 2-8°C	until expiry date; 24 months from date of production
conjugate	ready-to-use solution, at 2-8°C, Avoid contamination (sterile tips!)	until expiry date 28 months from date of production
washing solution	concentrate after opening at 2-8°C; working dilution at 2-8°C; working dilution at room temperature. Bottles used for the working dilution should be cleaned regularly, discard cloudy solutions.	until expiry date; 2 weeks; 1 week
dilution buffer	after opening at 2-8°C, Discard cloudy solutions!; unopened	24 months; until expiry date; 36 months from date of production
substrate	ready-to-use solution at 2-8°C, protected from light! Avoid contamination (sterile tips!) Discard when solution turns yellow (extinction against distilled water > 0.25).	until expiry date 24 months from date of production
stopping solution	after opening at room temperature	until expiry date

General Description

Leptospires are spiral-shaped, Gram-negative, obligate aerobic spirochetes with internal flagella. The genus is divided into two species, the pathogenic Leptospira interrogans and the free-living nonpathogenic Leptospira biflexa. Leptospira interrogans has about 200 different serovars based on the variability of surface antigens. Leptospires affect mammals (wild and domestic animals), reptiles and amphibians; they may be shed in the urine lifelong. Rats and other rodents are primary reservoirs for human infection. Infection is transmitted by urine-contaminated soil or water, rat bites or animal tissue. Especially occupational groups like agriculturists, plumbers, mine workers, fishermen and meatindustry workers are at great risk of exposure.
Mucosa and skin lesions are the most likely sites of entry for leptospires. Bacteria mainly proliferate in the central nervous system, kidneys and liver. The immune system removes organisms from blood and organs within 4-7 days by complement and humoral immunity. Cell-mediated immunity does not appear to be important. Leptospires within the convoluted tubules of the kidneys may survive due to the inefficiency of the immune system, the complement system in particular. For this reason infectious leptospires are shed in urine. Micro-agglutination tests, ELISAs and indirect fluorescence-antibody tests are most frequently used for serodiagnosis.

Citations