Native E.coli L-Asparaginase

Product Overview

L-Asparaginase was purified from E.coli ASI.357

Target

E.coli L-Asparaginase

Nature

Native

Tag/Conjugate

Unconjugated

Bio-activity

One IU of L- Asparaginase is defined as that amount of enzyme required to generate 1 μmol of ammonia per minute at pH 7.3 and 37°C.

Alternative Names

E.coli L-Asparaginase; L-Asparaginase

Purity

Greater than 96.0% as determined by SDS-PAGE.

Format

Lyophilized

Preservative

None

Storage

Lyophilized L-Asparaginase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution L-Asparaginase should be stored at 4°C between 2-7 days and for future use below -18°C.

Reconstitution

It is recommended to reconstitute the lyophilized L-Asparaginase in 18M-cm H2O at 1mg/ml.

Introduction

L-Asparaginase is an enzyme that depletes L-Asparagine "an important nutrient for cancer cells" resulting in cancer/tumor cell starvation. L-asparaginase is an anti-tumor agent derived from E.coli.,which can inhibit the growth of malignant cells. It is used mainly for the induction of remission in acute lymphoblastic leukaemia. Because of the lymph node origin of malignant B cells in Multiple Myeloma, L-Asparagine is an essential amino acid for their cell metabolism, and, consequently, L-Asparaginase may be of value in managing the disease.

Keywords

E.coli L-Asparaginase; L-Asparaginase