The integrin alpha 10 subunit: expression pattern, partial gene structure, and chromosomal localization
CYTOGENETICS AND CELL GENETICS
Authors: Lehnert, K; Ni, J; Leung, E; Gough, S; Morris, CM; Liu, D; Wang, SX; Langley, R; Krissansen, GW
Abstract
Herein we report the cloning of cDNAs and incompletely processed hnRNAs from endothelia and heart that encode the alpha 10 subunit forming part of the novel collagen type II-binding integrin alpha 10 beta 1 of chondrocytes. Analysis of hnRNA clones and reported expressed sequence tags revealed the positions of 17 putative intron-exon splice junctions shared with those of the p150,95(ITGAX) gene. Human alpha 10 transcripts of 5.4 and 1.8 kb were not restricted to chondrocytes bur, instead, were widely expressed in a panel of 24 tissue types, where the highest expression was found in muscle and heart. The human alpha 10 subunit gene (ITGA10) was localized to band q21 of chromosome 1. Copyright (C) 2000 S. Karger AG, Basel.
Epigenetic and in vivo comparison of diverse MSC sources reveals an endochondral signature for human hematopoietic niche formation
BLOOD
Authors: Reinisch, Andreas; Etchart, Nathalie; Thomas, Daniel; Hofmann, Nicole A.; Fruehwirth, Margareta; Sinha, Subarna; Chan, Charles K.; Senarath-Yapa, Kshemendra; Seo, Eun-Young; Wearda, Taylor; Hartwig, Udo F.; Beham-Schmid, Christine; Trajanoski, Slave; Lin, Qiong; Wagner, Wolfgang; Dullin, Christian; Alves, Frauke; Andreeff, Michael; Weissman, Irving L.; Longaker, Michael T.; Schallmoser, Katharina; Majeti, Ravindra; Strunk, Dirk
Abstract
In the last decade there has been a rapid expansion in clinical trials using mesenchymal stromal cells (MSCs) from a variety of tissues. However, despite similarities in morphology, immunophenotype, and differentiation behavior in vitro, MSCs sourced from distinct tissues do not necessarily have equivalent biological properties. We performed a genome-wide methylation, transcription, and in vivo evaluation of MSCs from human bone marrow (BM), white adipose tissue, umbilical cord, and skin cultured in humanized media. Surprisingly, only BM-derived MSCs spontaneously formed a BM cavity through a vascularized cartilage intermediate in vivo that was progressively replaced by hematopoietic tissue and bone. Only BM-derived MSCs exhibited a chondrogenic transcriptional program with hypomethylation and increased expression of RUNX3, RUNX2, BGLAP, MMP13, and ITGA10 consistent with a latent and primed skeletal developmental potential. The humanized MSC-derived microenvironment permitted homing and maintenance of long-term murine SLAM 1 hematopoietic stem cells (HSCs), as well as human CD34(+)/CD38(-)/CD90(+)/CD45RA(+) HSCs after cord blood transplantation. These studies underscore the profound differences in developmental potential between MSC sources independent of donor age, with implications for their clinical use. We also demonstrate a tractable human niche model for studying homing and engraftment of human hematopoietic cells in normal and neoplastic states.
COA
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