Intended Use
The kit has been verified by high purity Influenza H7N9 Hemagglutinin / HA recombinant protein.
The use of this kit for natural samples need be validated by the end user due to the complexity of natural targets and unpredictable interference.
Contents of Kit
1. Influenza H7N9 Hemagglutinin / HA Microplate: 96 well polystyrene microplate (12 strips of 8 wells) coated with rabbit mAb antibody against Influenza H7N9 Hemagglutinin / HA.
2. Influenza H7N9 Hemagglutinin / HA Detecion Antibody: 0.2 mg/mL of rabbit pAb antibody against Influenza H7N9 Hemagglutinin / HA conjugated to horseradish peroxidase (HRP) with preservatives.
3. Influenza H7N9 Hemagglutinin / HA Standard: Recombinant Influenza H7N9 (A/Shanghai/1/2013) Hemagglutinin / HA in a buffer with preservatives, lyophilized. The amount of standard is lot specific and indicated on the label of standard vial.
4. Wash Buffer Concentrate: 25 mL of a 20-fold concentrated solution of buffered surfactant with preservatives.
5. Dilution Buffer Concentrate: 8 mL of a 20-fold concentrated dilution buffer with preservatives.
6. Color Reagent A: 13 mL of stabilized hydrogen peroxide.
7. Color Reagent B: 13 mL of stabilized chromogen (tetramethylbenzidine).
8. Stop Solution: 8 mL of 2 N sulfuric acid.
Storage
Unopened Kit: Store at 2 - 8°C and the kit is stable for 6 months upon receipt.
Opened/Reconstituted Reagents:
Diluted Wash Buffer: Stored for up to 1 week at 2 - 8°C
Diluted Dilution Buffer: Stored for up to 1 week at 2 - 8°C
Conjugate: Stored for up to 1 month at 2 - 8°C
Stop Solution: Stored for up to 1 month at 2 - 8°C
Unmixed Color Reagent A: Stored for up to 1 month at 2 - 8°C
Unmixed Color Reagent B: Stored for up to 1 month at 2 - 8°C
Standard: After reconstitution, store for up to 1 month at -80°C. The reconstituted standards should be aliquoted and avoid repeated freeze-thaw cycles.
Microplate Wells: Return unused strips to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal. Stored for up to 1 month at 2 - 8°C
Sensitivity
The minimum detectable dose (MDD) of Influenza H7N9 Hemagglutinin / HA is typically less than 3 pg/mL. The MDD was determined by adding three standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
General Description
On April 1, 2013, the World Health Organization (WHO) first reported 3 human infections with a new influenza A (H7N9) virus in China. Since then, additional cases have been reported. Most reported cases have severe respiratory illness and, in some cases, have died. At this time, no cases of H7N9 outside of China have been reported. The new H7N9 virus has not been detected in people or birds in the United States.
This new H7N9 virus is an avian (bird) influenza (flu) virus. Human infections with avian influenza (AI, or "bird flu") are rare but have occurred in the past, most commonly after exposure to infected poultry. However, this is the first time that this bird flu subtype (H7N9) has been found in people. This virus is very different from other H7N9 viruses previously found in birds.
H7N9 viruses have been detected in poultry in the same area where human infections have occurred. Many of the human cases of H7N9 are reported to have had contact with poultry. However some cases reportedly have not had such contact. Close contacts of confirmed H7N9 patients are being followed to see if any human-to-human spread of H7N9 might have occurred. Based on previous experience with other avian influenza viruses – most notably H5N1 – some limited
human-to-human spread of this H7N9 virus would not be surprising. Most importantly, however, no sustained person-to-person spread of the H7N9 virus has been found at this time. Ongoing (sustained) person-to-person spread is necessary for a pandemic to occur.
The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer, and the influenza viral neuraminidase (NA) protein is a tetramer with an enzyme active site on the head of each monomer. Subtypes are further divided into strains; each genetically distinct virus isolate is usually considered to be a separate strain.
The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA0, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA0) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Standard Curve
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Citations
Publication ()
Have you cited DEIA-NS2501-5 in a publication?
Let us know and earn a reward for your research.
COA
Loading ......
