A DNA sequence encoding the Influenza A H5N1 virus (A/Hubei/1/2011(H5N1)) neuraminidase (AEO89183.1) (His36-Lys448) was fused with a polyhistidine tag at the N-terminus.
Nature
Recombinant
Tag/Conjugate
His
Molecular Weight
The recombinant influenza H5N1 virus neuraminidase (A/Hubei/2011 (H5N1)) comprises 433 amino acids and has a predicted molecular mass of 47.9 kDa. The apparent molecular mass of the recombinant protein is approximately 52 kDa in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 92 % as determined by SDS-PAGE
Format
Lyophilized from sterile PBS, pH 7.41. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -70°CStore it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Keywords
Influenzavirus A; Influenza A virus; Influenza A virus H5N1 NA;H5N1 NA; IAV H5N1 NA
Citations
Publication ()
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