Yellow Fever Virus IgG ELISA Kit

Sample

serum and plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

Enzyme ImmunoAssay (ELISA) for the determination of IgG antibodies to Yellow Fever Virus (YFV) in human plasma and sera coming from naturally infected people and vaccinated subjects to monitor the presence of protective IgG.

Contents of Kit

The kit contains reagents for the standard configuration of 96 tests.

1. Microplate: MICROPLATE
2. Negative Control CONTROL - 1×2.0ml/vial.
3. Positive Control CONTROL + 1×2.0ml/vial.
4. Wash buffer concentrate: WASHBUF 20× 1×60ml/bottle 20× concentrated solution.
5. Enzyme conjugate: CONJ 1×16ml/vial.
6. Chromogen/Substrate: SUBS TMB 1×16ml/vial.
7. Sulphuric Acid: H₂SO₄ 0.3 M 1×15ml/vial.
8. Specimen Diluent: DILSPE 2×60ml/vial.

Storage

2-8°C

Performance Characteristics

Evaluation of Performances has been conducted on selected panels collected both from (a) vaccinated recipients and healthy individuals living a still YFV almost free region, from (b) commercial suppliers of certified YFV IgG positive specimens and from (c) a random population of about 600 samples collected in a YFV-endemic country.
Sensitivity > 98 %
Specificity > 98 %

General Description

Yellow Fever virus is an RNA Flavivirus of the family of Flaviviridae transmitted by the mosquito "Aedes Aegypti", causing a severe infection in humans, quite often lethal if not immediately treated. Since many years a very efficient vaccine has been available and widely used to protect people living in endemic regions and travelers.
Diagnosis of YFV natural infection and follow-up of vaccination is generally obtained through detection of virus-specific IgG antibodies by means of ELISA techniques.