Sample
serum, plasma, cell culture supernatants, tissue homogenate
Detection Method
Sandwich ELISA
Intended Use
For quantitative detection of Human SLC25A6 (ADP/ATP translocase 3) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
0.156-10 ng/mL
Citations
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Nino-Soto, MI; Nuber, UA; et al. Differences in the pattern of X-linked gene expression between fetal bovine muscle and fibroblast cultures derived from the same muscle biopsies. CYTOGENETIC AND GENOME RESEARCH 111:57-64(2005).
Jeon, BG; Rho, GJ; et al. Low Levels of X-Inactive Specific Transcript in Somatic Cell Nuclear Transfer Embryos Derived from Female Bovine Freemartin Donor Cells. SEXUAL DEVELOPMENT 6:151-159(2012).