Human PSTK (L-seryl-tRNA (Sec) kinase) ELISA Kit

Aims: Paraquat Poisoning (PQ) can cause illness and death, and its main causes of mortality are acute respiratory failure and lung fibrosis. Early recognition of this condition and early treatment are vital. Thus, it is of importance to target the key genes controlling pathogenesis in the early stage of PQ. Main methods: C57BL/6 mice were used for Paraquat intragastric administration as a model of PQ Following a gene chip-based screening, the change of gene expression in the lung was further validated by bioinformatic analyses, co-expression network construction and real-time RT-PCR, Western blot and immunofluorescence assays. Key findings: 2287 genes with differential expression were identified at the very early stage of PQ From these, 76 genes that were linked to mitochondrion function were further pursued. Among these genes, PSTK was a phosphorylase kinase which serves a protective role in oxidative stress lung damage. PSTK was the central gene in a 30-gene network that is important for mitochondrial complex I assembly, mitochondrial apoptosis and mitochondrial fatty acid beta-oxidation, suggesting that they could conceivably be related to the pathogenesis of PQ induced lung damage. Lastly, we confirmed that PSTK was lowered in rodent lungs following PQ. Significance: PSTK emerges as a central gene in a network of mitochondrial function genes in PQ exposed mice. The functional role of PSTK in PQ induced lung injury warrants further examination. (C) 2015 Elsevier Inc. All rights reserved.

Styrene/tetradecyl methyl acrylate/3-methacryloxylpropyl trimethoxyl silane triblock copolymers (PSTKs), with well-defined structures and narrow molecular weight distributions, were synthesized by atom transfer radical polymerization. They were investigated as macromolecular coupling agents for the surface treatment of glass fibers. The reaction kinetics for the triblock copolymers were studied. The contact angles of the copolymers with water and diiodomethane showed that a modified-glass-fiber surface treated with a PSTK solution had strong hydrophobicity and that the impregnation of polypropylene on glass fibers was improved dramatically. In comparison with a film of 3-methacryloxylpropyl trimethoxyl silane, the polarity of the surface free energy of a PSTK film decreased, whereas the dispersion increased greatly. The critical concentration of the macromolecular coupling agents was obtained, and the monolayer saturated adsorptive capacity was calculated with the Gibbs absorption isotherm equation. (c) 2007 Wiley Periodicals, Inc.