Human NTs (Neurotensin) ELISA Kit

Name: Human NTs (Neurotensin) ELISA Kit
SKU: DEIA-FN1036
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

serum, plasma, cell culture supernatants, tissue homogenate

Species Reactivity

Human

Detection Method

Competitive ELISA

Intended Use

For quantitative detection of Human NTs (Neurotensin) in serum, plasma, tissue homogenates and other biological fluids.

Contents of Kit

1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 60 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Precision

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Detection Range

4.688-300 pg/mL

Sensitivity

2.813 pg/mL

Standard Curve

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Engineering nanotubular titania with gold nanoparticles for antibiofilm enhancement and soft tissue healing promotion

JOURNAL OF ELECTROANALYTICAL CHEMISTRY

Authors: Zheng, Xufeng; Sun, Jiao; Li, Wen; Dong, Biao; Song, Yanyan; Xu, Wenzhou; Zhou, Yanmin; Wang, Lin

Abstract

The success rate of titanium dental implants were challenged from peri-implant infections. However, pure titanium surfaces possess no antibacterial properties and are bio-inert for soft tissue healing delay. In the present study, the anodized titania nanotubes (TiO2-NTs) on Ti substrate were fabricated following by Au nanoparticle (AuNPs) deposition. The AuNPs incorporation into hierarchical TiO2-NTs would not only exhibit photocatalytic activity by visible-light irradiation, but also generate more quantum yield of reactive oxygen species, due to the prevention of electron-hole recombination and the surface plasmon resonance of Au, thus resulting in an enhanced antibacterial effect against multispecies biofilm. Antibacterial efficacy of AuNPs/TiO2-NTs was positively corelated with Au nanoparticle amount in TiO2-NTs samples. Besides, AuNPs decoration would also exert favorable functions on cell attachment, proliferation and migration of fibroblast. Experiments in animal model also indicated a superior soft tissue healing promotion for AuNPs/TiO2-NTs. Therefore, this designed AuNPs/TiO2-NTs is beneficial and applicable to dental implant surfaces to inhibit pathogens, promote soft tissue healing and combat periodontitis.

Changing epidemiology of invasive non -typhoid Salmonella infection: a nationwide population -based registry study

CLINICAL MICROBIOLOGY AND INFECTION

Authors: Mughini-Gras, L.; Pijnacker, R.; Duijster, J.; Heck, M.; Wit, B.; Veldman, K.; Franz, E.

Abstract

Objectives: Non -typhoid Salmonella (NTS) may invade beyond the intestine, causing bacteraemia, sepsis, and infection of normally sterile sites. The epidemiology of invasive NTS (iNTS) infection is under - researched. We determined trends, risk factors, serotype distribution, antimicrobial resistance (AMR), and attributable sources of iNTS infection in a high -income setting. Methods: 22,837 records of culture -con firmed human salmonellosis cases and 10,008 serotyped Sal- monella isolates from five putative animal reservoirs (pigs, cattle, broilers, layers, reptiles) in the Netherlands during 2005-2018 were retrieved from national surveillance registries. Risk factors for iNTS infection were identi fied using logistic regression analysis. Source attribution modelling was based on serotyping, prevalence, and exposure data. Results: The average annual percentage of iNTS infections was 4.6% (range 3.5-5.7%). An increase in iNTS infections was observed since 2012 (odds ratio (OR) 1.09, 95% con fidence interval (95% CI) 1.04-1.14). Increased iNTS infection risk was associated with wintertime (OR 1.37, 95% CI 1.12-1.66), male sex (OR 1.73, 95% CI 1.51-1.99), older age (ORs: 3.27 to 16.33, depending on age groups), and living in rural areas (OR 1.54, 95% CI 1.23-1.93). While 52% of iNTS infections (n = 950) were caused by serotypes Enteritidis and Typhimurium, those displaying the highest invasiveness relative to their occurrence were Dublin (32.9%, n = 163), Panama (21.6%, n = 106), and Poona (14.1%, n = 71). Cattle were a larger source of iNTS than non-iNTS infections (12.2% vs . 7.6%). Lower AMR and multi -resistance rates were observed among iNTS (37.9%) than non-iNTS isolates (48.6%). Discussion: The increase in iNTS infections, which is reported also in other countries, is of public health and clinical concern. The underlying reasons seem to be multi -factorial in nature. iNTS infection risk depends more on the infecting serotypes and patient demographics, and less on the attributable res- ervoirs and AMR pro files. L. Mughini-Gras, Clin Microbiol Infect 2020;26:941.e9 - 941.e14 ? 2019 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.