Human NRXN1(Neurexin-1)ELISA Kit

Sample

Serum, plasma, cell culture supernatant and other biological samples

Species Reactivity

Human

Detection Method

sELISA

Intended Use

In vitro quantitative determination of NRXN1 concentrations in serum, plasma, cell culture supernatant and other biological samples.

Contents of Kit

The sealed kit can be stored at 2-8 °C. The storage condition for opened kit is specified in the table below:
1. ELISA Microplate(Dismountable), 8×12. Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C
2. Lyophilized Standard, 2vial. Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C
3. Biotin-labeled Antibody(Concentrated, 100×), 120μl. 2-8°C (Avoid Direct Light)
4. HRP-Streptavidin Conjugate(SABC, 100×), 120μl. 2-8°C (Avoid Direct Light)
5. TMB Substrate, 10ml. 2-8°C (Avoid Direct Light)
6. Sample Dilution Buffer, 20ml. 2-8°C
7. Antibody Dilution Buffer, 10ml. 2-8°C
8. SABC Dilution Buffer, 10ml. 2-8°C
9. Stop Solution, 10ml. 2-8°C
10. Wash Buffer(25×), 30ml. 2-8°C
11. Plate Sealer, 5 pieces
12. Product Description, 1 copy
Note: The liquid reagent bottle contains slightly more reagent than indicated on the label. Please use pipette accurately measure and do proportional dilution

Storage

2-8°C (for sealed box), please do not freeze! See kit label for expiry date

Performance Characteristics

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Detection Range

78.125-5000 pg/ml

Sensitivity

46.875 pg/ml

General Description

Uniprot ID: Q9ULB1
Neurexin-1 is a presynaptic cell adhesion protein that mediates cell-cell interactions in the nervous system. It is predominantly expressed in neurons and plays critical roles in synapse formation, synaptic transmission, and neural circuit development. Neurexin-1 interacts with postsynaptic proteins, such as neuroligins, and extracellular matrix molecules, facilitating synaptic adhesion, neurotransmitter release, and synaptic plasticity in the brain and spinal cord.

Standard Curve

1. Calculate the mean OD450 value (using the original OD450 value or the corrected OD450 value) of the duplicate readings for each standard, control, and sample. Then, obtain the value of calculation by subtracting the OD450 blank.
2. Create a four parameter logistic curve by plotting the mean absorbance for each standard on the y-axis against the concentration on the x-axis. (Remove the OD450 blank during plotting.) Alternatively, you can use the curve fitting software offered by the microplate reader (e.g. Thermo SkanIt RE software, Curve Expert 1.3 or 1.4).
3. Calculate the sample concentration by substituting OD450 value into the standard curve. Diluted samples should be multiplied by the relevant dilution ratio.