Sample
serum, plasma, cell culture supernatants, tissue homogenate
Detection Method
Sandwich ELISA
Intended Use
For quantitative detection of Human NOTUM (Protein notum homolog) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
78-5000 pg/mL
Citations
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Lee, J; Garmestani, K; et al. In vitro and in vivo evaluation of structure-stability relationship of In-111- and Ga-67-labeled antibody via 1B4M or C-NOTA chelates. NUCLEAR MEDICINE AND BIOLOGY 24:225-230(1997).
Huclier-Markai, S; Sabatie, A; et al. Chemical and biological evaluation of scandium(III)-polyaminopolycarboxylate complexes as potential PET agents and radiopharmaceuticals. RADIOCHIMICA ACTA 99:653-662(2011).
COA
Certificate of Analysis
