Sample
serum, plasma, cell culture supernatants, tissue homogenate
Detection Method
Sandwich ELISA
Intended Use
For quantitative detection of Human LRP2 (Low-density lipoprotein receptor-related protein 2) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
0.78-50 ng/mL
Citations
Publication ()
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LRP2 is a potential molecular target for pathological myopia
Delaunay K, Picard E, Lassiaz P, Jonet L, Cannaya V, Ruiz-Moreno JM, Kojima K, Vorum H, Honoré B, Ruiz-Medrano J, Cehofski LJ, Pussard E, Kozyraki R, Torriglia A, Cases O, Behar-Cohen F
Applications: ELISA
Reactive species: Human
"Abstract: High myopia (HM) and posterior staphyloma (PS) are major causes of vision loss worldwide. Genetic and environmental factors, especially light exposure, influence myopia. This study shows that low-density lipoprotein-related receptor type 2 (LRP2) levels are decreased in the vitreous of patients with HM and PS, and that in human donor eyes affected by PS, LRP2 expression was reduced in the neural retina and retinal pigment epithelium (RPE), with morphologic changes similar to those observed in the Foxg1-Cre-Lrp2fl/fl mouse that also develops PS. In human induced pluripotent stem cell-derived RPE cells, LRP2 silencing regulated genes involved in eye and neuronal development, visual perception, tissue remodeling, hormone metabolism, and RPE structure. Its expression increased under light exposure, particularly red light, but was downregulated by cortisol. These findings establish a link between LRP2, myopization, and environmental factors, highlighting its crucial role in nonsyndromic HM and PS. LRP2 appears to be a promising therapeutic target for HM treatment."
Article snippet: ELISA protein quantification of LRP2. LRP2 concentration was determined by ELISA (Creative Diagnostics, DEIA-FN834). 
Figure 1. Concentrations of LRP2 protein in ng/mL in undiluted vitreous from control (n = 13) and NSHM (n = 10) eyes quantified with ELISA.
COA
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