Human L-Dihydroxyphenyalanine ELISA Kit

Name: Human L-Dihydroxyphenyalanine ELISA Kit
SKU: DEIA-NS2502-1
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Species Reactivity

Human

Detection Method

sELISA

Intended Use

This sandwich kit is for the accurate quantitative detection of Human L-Dihydroxyphenyalanine (also known as L-DOPA) in serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Standard Solution (3200ng/L)	0.5ml × 1	2-8°C
2	Pre-coated ELISA Plate	96T × 1	2-8°C
3	Standard Diluent	3ml × 1	2-8°C
4	Streptavidin-HRP	6ml × 1	2-8°C
5	Stop Solution	6ml × 1	2-8°C
6	Substrate Solution A	6ml × 1	2-8°C
7	Substrate Solution B	6ml × 1	2-8°C
8	Wash Buffer Concentrate (25×)	20ml × 1	2-8°C
9	Biotinylated Human L-DOPA Antibody	1ml × 1	2-8°C
10	User Instruction	1
11	Plate Sealer	2 pics
12	Zipper bag	1 pic

Storage

Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
CV(%) = SD/mean × 100%
Intra-Assay: CV<8%
Inter-Assay: CV<10%

Sensitivity

5.12ng/L

Standard Curve

This standard curve is only for demonstration purposes. A standard curve should be generated with each assay.