Specificity: Recognizes the third constant domain (CH3) of the alpha chain of human IgA and reacts with both IgA1 and IgA2 isotypes and not with other types of immunoglobulins.
Species Reactivity: Human
Application: ELISA, FC, IB, IHC, IP
Summary
Specifications
Antibody Isotype
IgG1k
Clone
HB2
Species Reactivity
Human
Immunogen
Human salivary proteins
Conjugate
Unconjugated
Applications
Application Notes
ELISA (solid phase: 0.1-100 μg/ml; tracer: 0.001-100 μg/ml for 30 min at RT) FC (0.5-1.0 μg/million cells in 0.1 ml) IB (1-2 μg/ml) IHC (1-2 μg/ml for 30 min at RT; staining of formalin-fixed tissues is served by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) IP (1-2 μg per 100-500 μg of total cell lysate protein/1 ml of anti mouse coated Sepharose-4B suspension)
Target
Alternative Names
IgA; Immunoglobulin A; IgA CH3
Citations
Publication ()
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