Recombinant Human IA2 [His]

Name: Recombinant Human IA2 [His]
SKU: DAGC269
Rating: 5 (1 reviews)

Human IA2 antigen, recombinant protein from insect cells

COA

SDS

Datasheet

Summary

Product Overview

cDNA coding for the amino acids 603 to 979 of human IA-2 (ICA 512) fused to a hexa-histidine purification tag. This corresponds to the intracellular region of ICA 512 and contains the sequence of the tryptic protein fragment originally defined as IA-2 autoantigen.

Nature

Recombinant

Tag/Conjugate

His

Molecular Weight

45 kDa

Alternative Names

IA2; IA-2 protein; tyrosine phosphatase ICA 512

Purity

> 80%

Format

Liquid

Buffer

Neutral to slightly alkaline pH and 20% glycerol as cryoprotective agent.

Preservative

None

Storage

Store at -70°C or below. Avoid repeated freeze-thaw cycles.

Introduction

Type 1 diabetes, also known as insulin-dependent diabetes mellitus (IDDM), results from a chronic autoimmune destruction of the insulin-secreting pancreatic beta cells, probably initiated by exposure of genetically susceptible host to an environmental agent. Autoimmune destruction of beta cells is thought to be completely asymptomatic until 80 -90% of the cells are lost. This process may take years to complete and may occur at any time. During the preclinical phase, this autoimmune process is marked by circulating autoantibodies to beta cell antigens. These autoantibodies are present years before the onset of type 1 diabetes and prior to clinical symptoms. Early studies utilized the immunofluorescence test for islet-cell antibodies (ICA), which has been difficult to standardize and is now replaced by a combination of several radioimmunoassays for antibodies against specific beta cell antigens, such as insulin (IAA), glutamic acid decarboxylase (GAD) and tyrosine phosphatase ICA 512 (IA2). IA2, a member of the protein tyrosine phosphatases family is localized in the dense granules of pancreatic beta cells and the second defined recombinant islet cell antigen. IA2 shares sequence identity with the islet cell antigen 512. The higher frequency of antibodies to IA2 is explained by the presence of autoantibodies directed to the COOH terminus of IA2 which is lacking in the ICA512 molecule. IA2 autoantibodies are present in the majority of individuals with new-onset type 1 diabetes and in individuals in the pre-diabetic phase of the disease. The appearance of autoantibodies to IA2 seems to be correlated with the rapid progression to overt type 1 diabetes. The combination of tests for GAD65 and IA2 autoantibodies is highly relevant for risk assessment of type 1 diabetes in children and adolescence. The screening for GAD65 and IA2 autoantibodies detect more than 90 % of subjects at risk for type 1 diabetes and may, therefore, possess the potential to replace ICA technique.

Keywords

IA-2; PTPRN; Islet Antigen-2; Type 1 Diabetes; T1D; Diabetes

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References

Laparoscopic radical hysterectomy with lymphadenectomy in patients with early cervical cancer: Our instruments and technique

SURGICAL ONCOLOGY-OXFORD

Authors: Malzoni, Mario; Tinelli, Raffaele; Cosentino, Francesco; Perone, Ciro; Iuzzolino, Domenico; Rasile, Marianna; Tinelli, Andrea

Abstract

The purpose of this study is to describe the technique of total laparoscopic radical hysterectomy (type III procedure) with lymphadenectomy as performed at the Advanced Gynecological Endoscopy Center of the Malzoni Medical Center, Avellino, Italy. Seventy-seven patients underwent total laparoscopic radical hysterectomy (type 11, 111) with lymphadenectomy between January 2000 and March 2008. FIGO stage included five patients Ia1 with LVSI (lymph-vascular involvement), 24 patients Ia2, and 48 patients Ib1. 60 patients underwent a class III procedure and 17 patients a class II procedure according to the Piver classification. Histological types included squamous cell carcinoma in 65 patients, adenocarcinomas in 10 patients, and adenosquamous carcinoma in two. Para-aortic lymphadenectomy was performed up to the level of the inferior mesenteric artery in eight cases with positive pelvic lymph nodes at frozen section evaluation. Total laparoscopic radical hysterectomy can be considered a safe and effective therapeutic procedure for the management of early stage cervical cancer with a low morbidity; moreover, the laparoscopic route may offer an alternative option for patients undergoing radical hysterectomy, although multicenter studies and tong-term follow-up are required to evaluate the oncologic outcomes of this procedure. (C) 2008 Elsevier Ltd. All rights reserved.

Improved expression and characterization of recombinant human Golgi alpha 1, 2-mannosidase I isoforms (IA2 and IC) by Escherichia coli

JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Authors: Isoyama-Tanaka, Junko; Dohi, Koji; Misaki, Ryo; Fujiyama, Kazuhito

Abstract

Golgi alpha 1,2-mannosidase I is involved in the N-linked oligosaccharide processing pathway. In this study, two truncated genes encoding for human Golgi alpha 1,2-mannosidase I (hManIA2: amino acids 127-626 and hManIC: amino acids 118-617) were expressed in Escherichia coli to characterize the enzymes. These genes were fused to a 17 protein tag and a histidine tag at the N- and C-terminal ends, respectively, and purified using Co2+ affinity chromatography. The properties including optimal temperature, optimal pH, and substrate specificity of the purified enzymes were investigated by HPLC using pyridylamino (PA)-labeled oligosaccharides as substrates. The stability of hManIA2 was dependent on the presence of Ca2+, which was also required for its activity. On the other hand, hManIC was stable in the absence of Ca2+, even though Ca2+ was also effective for the activity of hManIC. While the similarity of the amino acid sequences is over 60%, hManIA2 and hManIC showed different substrate specificities particularly toward M9A and M8C. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.