Serum, plasma, cell culture supernatant and other biological samples
Species Reactivity
Human
Detection Method
cELISA
Intended Use
In vitro quantitative determination of HS concentrations in serum, plasma, cell culture supernatant and other biological samples.
Contents of Kit
No.
Components
Size
Storage Conditions
1
ELISA Microplate(Dismountable)
96T(8×12)
2-8°C/-20°C
2
Lyophilized Standard
2vial
2-8°C/-20°C
3
Biotin-labeled Antibody (Lyophilized)
1vial
2-8°C/-20°C
4
HRP-Streptavidin Conjugate (SABC, 100×)
120 ul
2-8°C
5
TMB Substrate
10 ml
2-8°C
6
Purified water
200 ul
2-8°C
7
Sample Dilution Buffer
20 ml
2-8°C
8
Antibody Dilution Buffer
10 ml
2-8°C
9
SABC Dilution Buffer
10 ml
2-8°C
10
Stop Solution
10 ml
2-8°C
11
Wash Buffer (25×)
30 ml
2-8°C
12
Plate Sealer
5
-
Storage
Store at 2-8°C (for sealed box), please do not freeze! See kit label for expiry date. Stability Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate. Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Detection Range
78.125-5000ng/ml
Sensitivity
46.9ng/ml
Standard Curve
The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.
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