Human HbCO (Carboxyhemoglobin) ELISA Kit

Name: Human HbCO (Carboxyhemoglobin) ELISA Kit
SKU: DEIA-NS2408-3
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

serum, plasma, cell culture supernatant and other biological samples

Species Reactivity

Human

Detection Method

sELISA

Intended Use

In vitro quantitative determination of HbCO concentrations in serum, plasma, cell culture supernatant and other biological samples.

Contents of Kit

No.	Components	Size	Storage Conditions
1	ELISA Microplate(Dismountable)	8×12	Stored for 1 month at 2-8°C
2	Lyophilized Standard	2 vial	Stored for 1 month at 2-8°C
3	Biotin-labeled Antibody(Concentrated, 100×)	120μl	2-8°C (Avoid Direct Light)
4	HRP-Streptavidin Conjugate(SABC, 100×)	120μl	2-8°C (Avoid Direct Light)
5	TMB Substrate	10ml	2-8°C (Avoid Direct Light)
6	Sample Dilution Buffer	20ml	2-8°C
7	Antibody Dilution Buffer	10ml	2-8°C
8	SABC Dilution Buffer	10ml	2-8°C
9	Stop Solution	10ml	2-8°C
10	Wash Buffer(25×)	30ml	2-8°C
11	Plate Sealer	5 pieces
12	Product Description	1 copy

Storage

2-8°C (for sealed box), please do not freeze! See kit label for expiry date

Performance Characteristics

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Detection Range

31.25-2000ng/ml

Sensitivity

18.75ng/ml

Standard Curve

This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C - 25°C. TMB was balanced to 37°C before color development, and incubated at 37°C for 15 minutes in the dark after adding the enzyme label plate holes.)
The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.