Human CYP27B1 (25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial) ELISA Kit (DEIA-FN365)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma, cell culture supernatants, tissue homogenate
Species Reactivity
Human
Intended Use
For quantitative detection of Human CYP27B1 (25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
0.78-50 ng/mL
Sensitivity
0.469 ng/mL
Standard Curve

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References


Currenti, SA; et al. Understanding and Determining the Etiology of Autism. CELLULAR AND MOLECULAR NEUROBIOLOGY 30:161-171(2010).
Li, D; Wang, X; et al. Tumor-Produced Versican V1 Enhances hCAP18/LL-37 Expression in Macrophages through Activation of TLR2 and Vitamin D3 Signaling to Promote Ovarian Cancer Progression In Vitro. PLOS ONE 8:-(2013).

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