Human CTSG(Cathepsin G) ELISA Kit

Sample

Serum, plasma, cell culture supernatant and other biological samples

Species Reactivity

Human

Detection Method

sELISA

Intended Use

In vitro quantitative determination of CTSG concentrations in serum, plasma, cell culture supernatant and other biological samples.

Contents of Kit

The sealed kit can be stored at 2-8 °C. The storage condition for opened kit is specified in the table below:
1. ELISA Microplate(Dismountable), 8×12. Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C
2. Lyophilized Standard, 2vial. Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C.
3. Biotin-labeled Antibody(Concentrated, 100×), 120μl. 2-8°C (Avoid Direct Light)
4. HRP-Streptavidin Conjugate(SABC, 100×), 120μl. 2-8°C (Avoid Direct Light)
5. TMB Substrate, 10ml. 2-8°C (Avoid Direct Light)
6. Sample Dilution Buffer, 20ml. 2-8°C
7. Antibody Dilution Buffer, 10ml. 2-8°C
8. SABC Dilution Buffer, 10ml. 2-8°C
9. Stop Solution, 10ml. 2-8°C
10. Wash Buffer(25×), 30ml. 2-8°C
11. Plate Sealer, 5 pieces
12. Product Description, 1 copy
Note: The liquid reagent bottle contains slightly more reagent than indicated on the label. Please use pipette accurately measure and do proportional dilution.

Storage

2-8°C (for sealed box), please do not freeze! See kit label for expiry date

Performance Characteristics

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Detection Range

0.156-10ng/ml

Sensitivity

0.094ng/ml

General Description

Uniprot ID: P08311
Cathepsin G is a serine protease which was first identified in the cytoplasmic azurophilic granules of neutrophil leukocytes. As a degradative enzyme, Cathepsin G acts both intracellularly to degrade ingested host pathogens and extracellularly to breakdown ECM components at inflammatory sites. Independent of its serine protease activity, Cathepsin G is also known to exert broad-spectrum antibacterial action against Gram-negative and –positive bacteria. Cathepsin G has been reported to play an important role in the pathophysiology of several diseases, such as chronic obstructive pulmonary disease (COPD), rheumatoid arthritis, cystic fibrosis, and other conditions clinically manifested by excessive inflammatory reactions.

Standard Curve

This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C -25°C. TMB was balanced to 37°C before color development, and incubated at 37°C for 15 minutes in the dark after adding the enzyme label plate holes.)
The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.