Sample
serum, plasma, cell culture supernatants, tissue homogenate
Detection Method
Sandwich ELISA
Intended Use
For quantitative detection of Human BAG3 (Bcl2 Associated Athanogene 3) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
0.156-10 ng/mL
Citations
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Q & A
Q: Inquiry for the information of antibodies used in the kit - capture,detect, clone.
A: Capture: Mouse monoclonal antibody
Detect: Rabbit polyclonal antibody
Antigen: O95817:2-575aa
Customer Reviews
Yoon, JS; Lee, MY; et al. Bis Is Involved in Glial Differentiation of P19 Cells Induced by Retinoic Acid. KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY 13:251-256(2009).
De Marco, M; Falco, A; et al. Detection of soluble BAG3 and anti-BAG3 antibodies in patients with chronic heart failure. CELL DEATH & DISEASE 4:-(2013).