Human Anti-Soluble Liver Antigen IgG Antibody ELISA Kit

Sample

Serum, plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The Human Anti-Soluble Liver Antigen IgG Antibody ELISA Kit is designed for the Semi-quantitative analysis of Human Serum, plasma samples.

Contents of Kit

1. Polystyrene microwell ELISA plates coated with a purified antigen(12-1 x 8 wells), with holder in foil package containing desiccants
2. Negative Control, 1 vial of buffer 1.2 mL
3. Positive Control, 1 vial of buffer 1.2 mL
4. Calibrator A, 1 vial of buffer containing preservative, prediluted, 1.2 mL
5. Calibrator B, 1 vial of buffer containing preservative, prediluted, 1.2 mL
6. Calibrator C, 1 vial of buffer containing preservative, prediluted, 1.2 mL
7. Calibrator D, 1 vial of buffer containing preservative, prediluted, 1.2 mL
8. Sample Diluent, 1 vial – colored straw containing PBS-buffered saline, protein stabilizers and preservative, 50 mL.
9. Antibody Enzyme Conjugate, colored blue containing buffer, protein stabilizers and preservative, 12 mL
10. Wash Buffer (20 ×), 50 mL
11. TMB Chromogen, containing stabilizers, 10 mL
12. Stop Solution, Colorless, 10 mL

Storage

2–8 °C, protected from light and moisture.

Detection Range

0 - 200 U

General Description

Anti-soluble liver antigen (SLA) antibodies are autoantibodies primarily associated with autoimmune hepatitis (AIH), particularly AIH type 1, and can be a marker for the disease in patients where other antibodies are absent. These antibodies are directed against a UGA-suppressor tRNA associated protein and their presence suggests the need for immunosuppressive treatment, as they are linked to higher rates of relapse after steroid therapy.