Contents of Kit
1. Polystyrene microwell ELISA plates coated with a purified antigen(12-1 x 8 wells), with holder in foil package containing desiccants
2. Negative Control, 1 vial of buffer 1.2 mL
3. Positive Control, 1 vial of buffer 1.2 mL
4. Calibrator A, 1 vial of buffer containing preservative, prediluted, 1.2 mL
5. Calibrator B, 1 vial of buffer containing preservative, prediluted, 1.2 mL
6. Calibrator C, 1 vial of buffer containing preservative, prediluted, 1.2 mL
7. Calibrator D, 1 vial of buffer containing preservative, prediluted, 1.2 mL
8. Sample Diluent, 1 vial – colored straw containing PBS-buffered saline, protein stabilizers and preservative, 50 mL.
9. Antibody Enzyme Conjugate, colored blue containing buffer, protein stabilizers and preservative, 12 mL
10. Wash Buffer (20 ×), 50 mL
11. TMB Chromogen, containing stabilizers, 10 mL
12. Stop Solution, Colorless, 10 mL
General Description
Anti-Melanocyte Melan-A Antibody is a monoclonal antibody that targets the Melan-A (MART-1) protein. Melan-A is a 20–22 kDa transmembrane protein predominantly expressed in melanocytes (pigment-producing cells) and melanoma cells. It is a highly specific marker for the melanocytic lineage, making it a valuable tool in diagnostic pathology.
Anti-Melan-A antibody is highly specific for melanocytic cells, making it a reliable marker for diagnosing both primary and metastatic melanomas. It stains melanoma cells in over 80% of cases, providing a strong diagnostic signal.
This antibody is positive in benign melanocytic lesions (such as nevi) and helps differentiate them from non-melanocytic tumors. It is particularly useful in distinguishing melanoma from other spindle cell tumors that may lose expression of other markers like gp100 (HMB-45).
Citations
COA
Certificate of Analysis
