Recombinant HPV16 E7 Protein [His]

Name: Recombinant HPV16 E7 Protein [His]
SKU: DAGC276
Rating: 5 (1 reviews)

Recombinant HPV16 E7 Protein from E.coli [His]

COA

SDS

Datasheet

Summary

Product Overview

Recombinant Human HPV16 E7 produced in E. coli aa 1-98 with 6His, N-terminus tag(s).

Nature

Recombinant

Tag/Conjugate

His

Molecular Weight

15.0 kD

Endotoxin

Less than 1.0 EU/μg protein (determined by LAL method).

Alternative Names

HPV16 E7; HPV type 16 E7; HPV16

Format

Liquid

Buffer

PBS with 8M Urea

Preservative

None

Storage

Store at 2-8°C for short term. Store at -20°C for long term. Avoid multiple freeze/thaw cycles

Reconstitution

Briefly centrifuge vial prior to opening. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. Add 5-50% of glycerol (final concentration).

Introduction

The Papillomavirus article covers the general biological features of human and animal papillomaviruses. Human papillomavirus (HPV) is a virus from the papillomavirus family of viruses that is capable of infecting humans. Like all papillomaviruses, HPVs establish productive infections only in keratinocytes of the skin or mucous membranes. While the majority of the nearly 200 known types of HPV cause no symptoms in most people, some types can cause warts (verrucae), while others can – in a minority of cases – lead to cancers of the cervix, vulva, vagina, and anus in women or cancers of the anus and penis in men. It can also cause cancers of the head and neck (tongue, tonsils and throat). Recently, HPV has been linked with an increased risk of cardiovascular disease.

Keywords

HPV16 E7; HPV type 16 E7; HPV16

Citations

Have you cited DAGC276 in a publication? Let us know and earn a reward for your research.

Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

HPV16 E6 and E7 upregulate the histone lysine demethylase KDM2B through the c-MYC/miR-146a-5p axys

ONCOGENE

Authors: Peta, Elektra; Sinigaglia, Alessandro; Masi, Giulia; Di Camillo, Barbara; Grassi, Angela; Trevisan, Marta; Messa, Lorenzo; Loregian, Arianna; Manfrin, Erminia; Brunelli, Matteo; Martignoni, Guido; Palu, Giorgio; Barzon, Luisa

Abstract

Persistent infection by high-risk human papillomaviruses (HPVs) is associated with the development of cervical cancer and a subset of anogenital and head and neck squamous cell carcinomas. Abnormal expression of cellular microRNAs (miRNAs) plays an important role in the development of cancer, including HPV-related tumors. In this study, we demonstrated that miR-146a-5p was down-regulated by E6 and, less efficiently, by E7 of high-risk HPV16 in keratinocytes and the presence of low levels of this miRNA in cervical carcinoma cell lines and in high-risk HPV-positive cervical specimens. Downregulation of miR-146a-5p was mediated at least in part by the transcription repressor c-MYC, through binding sites in the miR-146a promoter. Overexpression of miR-146a-5p significantly inhibited proliferation and migration of keratinocytes and cervical cancer cells. The histone demethylase KDM2B was validated as a new direct target of miR-146a-5p and two putative binding sites for miR-146a-5p were identified in its 3'UTR sequence. Western blot analysis and immunohistochemistry showed that KDM2B was overexpressed in HPV16 E6/E7-positive keratinocytes, in cervical cancer cell lines, and in a subset of invasive cervical carcinomas and HPV-positive laryngeal squamous cell carcinomas. In these tumors, KDM2B overexpression was associated with c-MYC copy number gain. In vitro, silencing of KDM2B inhibited proliferation of cervical cancer cells. In conclusion, this study identified a novel player, the hystone demethylase KDM2B, in HPV-mediated tumorigenesis. E6 and, less efficiently, E7 of high-risk HPV16 up-regulated KDM2B expression in human keratinocytes through a pathway involving overexpression of c-MYC, which in turn downregulated miR-146a-5p.

Synthesis of Proteins Encoded by the Early Genes E2, E6, and E7 of Papillomavirus of Type 16 in the Plant Expression System

DOKLADY BIOCHEMISTRY AND BIOPHYSICS

Authors: Salyaev, R. K.; Rekoslavskaya, N. I.; Stolbikov, A. S.

Abstract

An expression system for the synthesis of early antigenic proteins HPV16 E2, HPV16 E6, and HPV16 E7 of high-risk papillomavirus based on transgenic tomato fruits was developed. It is planned to use the early antigenic proteins synthesized to create a therapeutic vaccine against cervical cancer.