Rabbit Anti-Human HPL polyclonal antibody

Universiti Teknologi PETRONAS (UTP) has formed a high performance computing cluster (HPCC) inside the campus by utilizing most of the campus' existing resources as the computing resources. All the resources are interconnected within a same network, enabling it to be accessed by users anywhere anytime within and outside the campus. The UTP HPCC is used by researchers, including lecturers and students from UTP also from external parties to compute intensive applications. However, the UTP HPCC has never been benchmarked before, hence the performance and the ability of UTP HPCC are still unverified. The benchmarking is imperative in order to measure the true performance and the ability of UTP HPCC. This paper aims to evaluate the performance of UTP HPCC using a suitable benchmarking tool as well as to determine the optimal parameters configuration of the selected benchmarking tool. A comparative study has been done in order to select the best benchmarking tool between High performance LINPACK (HPL) and the NAS Parallel. The results show that HPL is a more suitable benchmarking tool for UTP HPCC compared to NAS Parallel. A series of experiments were carried out to select the optimum parameter of HPL configuration for UTP HPCC. The results from benchmarking show that the peak performance is achievable under the test conditions.

Background: Osteoclasts play a critical role in bone resorption due to orthodontic tooth movement (OTM). In OTM, a force is exerted on the tooth, creating compression of the periodontal ligament (PDL) on one side of the tooth, and tension on the other side. In response to these mechanical stresses, the balance of receptor activator of nuclear-factor kappa-B ligand (RANKL) and osteoprotegerin (OPG) shifts to stimulate osteoclastogenesis. However, the mechanism of OPG expression in PDL cells under different mechanical stresses remains unclear. We hypothesized that compression and tension induce different microRNA (miRNA) expression profiles, which account for the difference in OPG expression in PDL cells. To study miRNA expression profiles resulting from OTM, compression force (2 g/cm(2)) or tension force (15% elongation) was applied to immortalized human PDL (HPL) cells for 24 h, and miRNA extracted. The miRNA expression in each sample was analyzed using a human miRNA microarray, and the changes of miRNA expression were confirmed by real-time RT-PCR. In addition, miR-3198 mimic and inhibitor were transfected into HPL cells, and OPG expression and production assessed. Results: We found that certain miRNAs were expressed differentially under compression and tension. For instance, we observed that miR-572, - 663, - 575, - 3679-5p, UL70-3p, and - 3198 were upregulated only by compression. Real-time RT-PCR confirmed that compression induced miR-3198 expression, but tension reduced it, in HPL cells. Consistent with previous reports, OPG expression was reduced by compression and induced by tension, though RANKL was induced by both compression and tension. OPG expression was upregulated by miR-3198 inhibitor, and was reduced by miR-3198 mimic, in HPL cells. We observed that miR-3198 inhibitor rescued the compression-mediated downregulation of OPG. On the other hand, miR-3198 mimic reduced OPG expression under tension. However, RANKL expression was not affected by miR-3198 inhibitor or mimic. Conclusions: We conclude that miR-3198 is upregulated by compression and is downregulated by tension, suggesting that miR-3198 downregulates OPG expression in response to mechanical stress.