Magic™ Mouse Anti-GBS Ⅱ monoclonal antibody, clone J7221

Dosidicus gigas(D. gigas) is a commercially important squid species in the Eastern Pacific Ocean. Due to the wide distribution of the species and its variable biological characteristics, there is a lack of reliable information regarding its genetic structure, which restricts the stock assessment and management of theD. gigasfishery resource. Therefore, there is an urgent need to develop sufficient molecular markers for the conservation and management of this species. In this study, 101 novel single nucleotide polymorphism (SNP) markers were developed using genotyping-by-sequencing (GBS). The observed heterozygosity (H-o) and expected heterozygosity (H-e) ranged from 0.0000 to 1.0000 and 0.0340 to 0.5000, respectively. The polymorphism information content ranged from 0.0333 to 0.3750. Twenty-five loci significantly deviated from the Hardy-Weinberg equilibrium after a Bonferroni correction (p < 0.001). These polymorphic SNPs will be useful for further population genetic analyses forD. gigas.

The tribe Aethionemeae is sister to all other crucifers, making it a crucial group for unraveling genome evolution and phylogenetic relationships within the crown group Brassicaceae. In this study, we extend the analysis of Brassicaceae genomic blocks (GBs) toAethionemawhereby we identified unique block boundaries shared only with the tribe Arabideae. This was achieved using bioinformatic methods to analyze synteny between the recently updated genome sequence ofAethionema arabicumand other high-quality Brassicaceae genome sequences. We show that compared to the largely conserved genomic structure of most non-polyploid Brassicaceae lineages, GBs are highly rearranged inAethionema. Furthermore, we detected similarities between the genomes ofAethionemaandArabis alpina, in which also a high number of genomic rearrangements compared to those of other Brassicaceae was found. These similarities suggest that tribe Arabideae, a clade showing conflicting phylogenetic position between studies, may have diverged before diversification of the other major lineages, and highlight the potential of synteny information for phylogenetic inference.