Feline Leukemia Virus (FeLV) Antigen ELISA Kit

Sample

Serum, plasma, saliva, and bone marrow

Species Reactivity

Feline

Detection Method

sELISA

Intended Use

Feline Leukemia Virus (FeLV) ELISA Kit is an enzyme immunoassay kit for the qualitative Feline Leukemia Virus in cat serum, plasma, saliva, and bone marrow.

Contents of Kit

1. Antibody-coated microplate, 8 × 12 strips, 4°C. Unused strips should be sealed tightly in the air-tight pouch.
2. Control A (Negative Control), 2ml (Ready-to-use), 4°C
3. Control B (Cut-off Standard), 3ml (Ready-to-use), 4°C
4. Control C (Positive Control), 2ml (Ready-to-use), 4°C
5. HRP-Streptavidin solution, 20 ml (Ready-to-use), 4°C
6. Sample Diluent Buffer, 100 ml (Ready-to-use), 4°C
7. 20× Wash Buffer, 50 ml, 4°C
8. TMB Substrate, 15 ml, (Ready-to-use), 4°C (Protect from light)
9. STOP Solution, 15 ml, (Ready-to-use), 4°C
10. Plate sealer, 1 e.a., Room temperature

Storage

Store the unopened kit at 2-8 °C. Use the kit before expiration date.

Precision

The CV values of intra-assay precision is 6.7% and inter-assay precision is 8.6%.

Sensitivity

The diagnostic sensitivity is defined as the probability of the assay of scoring positive in the presence of the specific analytic.
It is 100% (95% confidence interval: 93.73% - 100%)

General Description

Feline leukemia virus (FeLV) is a retrovirus that infects cats. FeLV can be transmitted from infected cats when the transfer of saliva or nasal secretions is involved. If not defeated by the animal's immune system, the virus weakens the cat's immune system, which can lead to diseases which can be lethal. Because FeLV is cat-to-cat contagious, FeLV+ cats should only live with other FeLV+ cats. FeLV is categorized into four subgroups, A, B, C and T. An infected cat has a combination of FeLV-A and one or more of the other subgroups. Symptoms, prognosis and treatment are all affected by subgroup.