Human Fibulin-1 ELISA Kit

Sample

plasma, cell culture medium, other biological media

Species Reactivity

Human

Intended Use

This kit is used for the quantitative measurement of human Fibulin-1 in plasma, cell culture supernatant and other biological media.

Storage

- Upon receipt store all components at 4°C.
- Don't expose reagents to excessive light.

Precision

Intra-assay (Within-Run, n=7) CV=4.5-5.1 %
Inter-assay (Run-to-Run, n=5) CV=4.1-9.1 %

Sensitivity

The limit of detection (defined as such a concentration of human Fibulin-1 giving absorbance higher than mean absorbance of blank* plus three standard deviations of the absorbance of blank: A blank + 3SD blank) is better than 0.23 ng/mL of sample.

General Description

Fibulin-1 is the first described member of a seven-gene family of extracellular matrix (ECM) proteins that have a common structural signature consisting of a series of repeated EGF-like domains followed by a fibulin-type module at its carboxyl terminus. Fibulin-1 is a secreted glycoprotein that is an intercellular component of a wide range of connective tissues. In blood vessel walls, fibulin-1 is a component of elastic lamina and ECM fibers that surround smooth muscle cells and underlie the endothelium. Although its actual function remains to be elucidated, fibulin-1 has been suggested to be involved in cell adhesion, migration, proliferation, and malignant transformation through binding to many ECM proteins, including laminin, fibrinogen, fibronectin, nidogen-1, endostatin, β-amyloid precursor protein and proteoglycans, aggrecan, versican, receptors and growth factors. In addition, fibulin-1 has been found to bind to the plasma protein fibrinogen and to incorporate into fibrin clots formed in vitro and in vivo. Targeted inactivation of fibulin-1 gene in mice caused dilation and ruptures in the endothelial lining of small blood vessels, indicating that fibulin-1 was important in the stabilization of blood vessel walls. Fibulin-1 is one of a few ECM proteins normally found in blood in high concentrations. It was reported that plasma fibulin-1 concentrations increased in T2DM patients with prevalent cardiovascular disease destined for vascular surgery as well as in asthmatics.