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Human Papillomavirus (HPV) is a widespread group of viruses, with over 100 different types identified. Among them, HPV16 is recognized as one of the high-risk types, strongly associated with the development of various cancers, particularly cervical cancer, as well as anal, vaginal, vulvar, penile, and oropharyngeal cancers. The E7 oncoprotein encoded by HPV16 plays a pivotal role in the carcinogenic process. It disrupts the normal function of the pRb (retinoblastoma) tumor suppressor protein, leading to uncontrolled cell proliferation and the initiation of malignant transformation.
Human papillomavirus (HPV) is a group of small, non enveloped double stranded DNA viruses that can infect the skin and mucous membranes. HPV is classified into high-risk and low-risk types based on its carcinogenic potential. Among all high-risk HPV types, HPV-16 is the most common and dangerous type, associated with approximately 50% of cervical cancer cases worldwide, as well as the majority of anal, vulvar, vaginal, penile, and oropharyngeal cancers.
E7 is a key oncogenic protein encoded by the HPV-16 genome. It works together with another carcinogenic protein E6 and plays a central role in the virus driven cellular carcinogenesis process. The E7 protein hijacks the normal regulatory pathways of host cells through a series of complex molecular mechanisms, causing unlimited proliferation and ultimately leading to the formation of malignant tumors.
Figure 1. Structure of Human Papillomavirus 16.
Sample Type Compatibility
Sensitivity Profile
Kit Features
Key Features and Benefits
Technical Specifications
Components Provided
Cancer Research
It is extensively used in studies investigating the role of HPV16 E7 oncoprotein in cancer development. Researchers can use the kit to measure the expression levels of E7 in different cell lines, tissues, and animal models, helping to elucidate the molecular mechanisms underlying HPV-induced carcinogenesis.
Therapeutic Efficacy Evaluation
In the development and testing of new therapies targeting HPV16-related cancers, the kit is useful for evaluating the efficacy of the treatment. By measuring the levels of E7 oncoprotein before and after treatment, researchers and clinicians can determine whether the therapy is effective in reducing the expression of this oncoprotein, which is a key indicator of therapeutic success.
| Parameter | Details |
| Product name | Human HPV 16 E7 Oncoprotein ELISA Kit |
| Sample | Cell lysates, tissue lysates, cervical smears, plasma, serum |
| Intended Use | Human HPV16 E7 Oncoprotein ELISA Kit is an enzyme immunoassay developed for detection and quantitation of the Human Papillomavirus Type 16 E7 protein from cell lysates, tissue lysates, cervical smears, plasma and serum. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and HPV16 E7 samples. |
| Sensitivity | The kit has detection sensitivity limit of 31 pg/mL HPV16 E7. |
| General Description | Human papillomavirus (HPV) is a large group of more than 150 related DNA viruses. HPV is named for the warts (papillomas) some types can cause and is spread through direct skin-to-skin contact. Although most HPV infections cause no symptoms and resolve spontaneously, some persist and result in warts or precancerous lesions. Of the more than 150 types, over 40 types are known to be transmitted through sexual contact and make it the most commonly sexually transmitted infection (STI). |
| Standard Curve |  |
The Significance Of Studying E7 Oncogenic Protein
The in-depth study of HPV-16 E7 oncogenic protein is of great significance:
High Specificity
Utilizes highly specific monoclonal antibodies that show no significant cross-reactivity with other HPV types (e.g., HPV 18, 31, 33) or other common biological interferents. This ensures you are measuring HPV 16 E7 specifically.
Comprehensive Analysis
The kit includes a full set of standards for generating a standard curve, allowing for precise quantitative analysis rather than just qualitative detection. All reagents, including standards, are provided pre-formulated and optimized for convenience.
Accuracy
The accuracy of the kit is evaluated by spiking known concentrations of HPV16 E7 oncoprotein into different biological matrices (such as serum, cervical swab extracts). The recovery rate indicating that the kit can accurately quantify the E7 oncoprotein in various sample types.
In conclusion, the ELISA Kit for Human Papillomavirus 16 E7 Oncoprotein is a valuable tool for the detection and quantification of HPV16 E7 oncoprotein. With its high sensitivity, specificity, and reliability, it serves as an important asset in both research and clinical settings, contributing to the understanding, diagnosis, and treatment of HPV16-related cancers.
Lyse cells in a suitable non-denaturing lysis buffer (e.g., RIPA buffer) supplemented with protease inhibitors. Centrifuge the lysate at 10,000-15,000 x g for 10-15 minutes to remove insoluble debris. The resulting supernate should be diluted appropriately in the provided assay diluent before use in the ELISA.
The sample must be further diluted in the provided assay diluent and re-run. We recommend testing several dilutions of a new sample type to ensure the result falls within the linear range of the standard curve for accurate quantification.
The 96-well format is amenable to medium-throughput screening. For true HTS, please contact us to discuss automated liquid handling compatibility and potential bulk purchasing options.
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