Human HPV 16 E7 Oncoprotein ELISA Kit (DEIASL171)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Cell lysates, tissue lysates, or cervical smears
Species Reactivity
Intended Use
Human HPV16 E7 Oncoprotein ELISA Kit is an enzyme immunoassay developed for detection and quantitation of the Human Papillomavirus Type 16 E7 protein from cell lysates, tissue lysates, or cervical smears. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and HPV16 E7 samples.
Upon receiving, aliquot and store recombinant HPV16 E7 Standard at -20°C and avoid freeze/thaw.
Store all other components at 4°C.
Detection Range
The kit has detection sensitivity limit of 78 pg/mL HPV16 E7.
General Description
Human papillomavirus (HPV) is a large group of more than 150 related DNA viruses. HPV is named for the warts (papillomas) some types can cause and is spread through direct skin-to-skin contact. Although most HPV infections cause no symptoms and resolve spontaneously, some persist and result in warts or precancerous lesions. Of the more than 150 types, over 40 types are known to be transmitted through sexual contact and make it the most commonly sexually transmitted infection (STI).
HPV-induced cancers arise when viral sequences are integrated into the DNA of host cells. Some genes carried by the HPV virus, such as genes E6 and E7, act as oncogenes that promote tumor growth and malignant transformation. Roughly 12 HPV types (including 16, 18, 31, and 45) are classified as high-risk for being linked to malignancies. Nearly all cases of cervical cancer are associated with HPV infection, with two types present in 70% of cases: HPV16 and HPV18. Previous studies suggest that high-risk E7 oncoproteins are necessary for this cancer, by inactivating cell-cycle regulatory proteins. The ability to monitor these E7 levels may be a useful tool in cervical cancer screening and detection.


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Early Detection of Human Papillomavirus-Driven Oropharyngeal Cancer Using Serology From the Study of Prevention of Anal Cancer


Authors: Waterboer, Tim; Brenner, Nicole; Gallagher, Richard; Hillman, Richard John; Jin, Fengyi; Grulich, Andrew; Poynten, Isobel Mary

This case series examines HPV seropositivity and antibody levels in a cohort of older men who have sex with men.

Association of human papillomavirus vaccination with exposure to dental or medical visits


Authors: Shukla, Anubhuti; McKenna, Maria; Hayes, Catherine; Klevens, Ruth Monina

Background Human papillomavirus (HPV) infection is associated with oropharyngeal cancers. The Centers for Disease Control and Prevention (CDC) estimate that >15,000 new cases of HPV-associated oropharyngeal cancers are diagnosed in the United States annually. We evaluated an association between HPV vaccination and dental visits in the previous year. Methods Data were analyzed from the 2012, 2014, and 2016 Massachusetts Behavioral Risk Factor Surveillance System (MA-BRFSS) datasets. We created four categories of exposures to healthcare services in the past 12 months: a) both medical and dental visits, b) medical visit only, c) dental visit only, d) neither. Outcomes were HPV vaccination ever or influenza vaccination within the past 12 months. Logistic regression, controlled for race and education, was used to measure the association between medical/dental visits and vaccination status. Separate models were generated by sex. Results Crude and adjusted odds ratio of influenza and HPV vaccination were highest among males and females with both medical and dental visits. Women with both medical and dental provider visits had 3.7 times higher odds of being vaccinated for influenza and 1.7 times higher odds of being vaccinated for HPV. There were no differences in crude or adjusted odds among both males and females if the type of healthcare visits were only medical or only dental. Conclusion No difference in association between vaccination and medical or dental healthcare exposures suggests that oral health professionals might partner in promotion of positive health behaviors, including HPV vaccination. The type of provider did not affect the outcome as per this study.

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