Mouse anti-Human DENR monoclonal antibody, clone 2I4

TMA20 (MCT-1), TMA22 (DENR) and TMA64 (eIF2D) are eukaryotic translation factors involved in ribosome recycling and re-initiation. They operate with P-site bound tRNA in post-termination or (re-) initiation translation complexes, thus participating in the removal of 40S ribosomal subunit from mRNA stop codons after termination and controlling translation re-initiation on mRNAs with upstream open reading frames (uORFs), as well as de novo initiation on some specific mRNAs. Here we report ribosomal profiling data of S.cerevisiae strains with individual deletions of TMA20, TMA64 or both TMA20 and TMA64 genes. We provide RNA-Seq and Ribo-Seq data from yeast strains grown in the rich YPD or minimal SD medium. We illustrate our data by plotting differential distribution of ribosomal-bound mRNA fragments throughout uORFs in 5'-untranslated region (5' UTR) of GCN4 mRNA and on mRNA transcripts encoded in MAT locus in the mutant and wild-type strains, thus providing a basis for investigation of the role of these factors in the stress response, mating and sporulation. We also document a shift of transcription start site of the APC4 gene which occurs when the neighboring TMA64 gene is replaced by the standard G418-resistance cassette used for the creation of the Yeast Deletion Library. This shift results in dramatic deregulation of the APC4 gene expression, as revealed by our Ribo-Seq data, which can be probably used to explain strong genetic interactions of TMA64 with genes involved in the cell cycle and mitotic checkpoints. Raw RNA-Seq and Ribo-Seq data as well as all gene counts are available in NCBI Gene Expression Omnibus (GEO) repository under GEO accession GSE122039 (htips://wwvv.ncbLnim.nih.gov/geo/guery/acc.cgi?acc=GSE122039). (C) 2019 The Authors. Published by Elsevier Inc.

According to the Philippines' Department of Environment and Natural Resources (DENR), Metro Manila produces about 25% of the country's solid waste, 16% of which are plastics. Unfortunately, recycling is limited to only similar to 20% of these plastic wastes; the rest goes to landfills. To address this problem, the Humanitarian Engineering, Entrepreneurship, and Design (HEED) group of the University of the Philippines Diliman (UPD) started Project Plastikan - a plastics recycling project that aims to provide income opportunities for women in marginalized communities by building a plastics recycling facility based on the design of David Hakkens, founder of Precious Plastics. The facility will house various recycling machines with open-source designs to be built by UP students in partnership with the UP FABLAB. The pilot community is in Malabon, Metro Manila - one of the field sites of the UP College of Social Work and Community Development (CSWCD). The project involves three phases: (1) needs assessment and market research, (2) fabrication of appropriate machines, and (3) technology transfer and training. Through this project, the women in the community will be manufacturing upcycled products themselves. Aside from finished goods, useful raw materials may also be made and supplied to local manufacturers to turn into high-value products. Furthermore, the project will inculcate gender equality and empowerment among its beneficiaries, while providing a more holistic educational experience to the students and faculty members involved in the project.