A synthetic peptide corresponding to amino acid sequence 277-294 of rainbow trout (Oncorhynchus mykiss) CYP1A, coupled to the carrier protein keyhole limpet hemocyanin (KLH).
Conjugate
Unconjugated
Applications
Application Notes
ELISA: 1:300 - 1:1000; Western blot: 1:50 - 1:500 The monoclonal antibody C10-7 works well in both ELISA and western blot for a number of species. The antibody also works well in immunohistochemistry. Since assay conditions vary, the optimum dilution should be determined for each particular application. Note: The antibody is dissolved in a buffer containing BSA and is therefore not recommended to use for coating.
General Notes
This product consists of affinity-purified mouse monoclonal antibodies against peptide 277-294 of rainbow trout cytochrome P450 1A (CYP1A). The monoclonal antibody cross-reacts with CYP1A in liver samples from a variety of fish species, including rainbow trout (Oncorhynchus mykiss), Atlantic salmon (Salmo salar), carp (Cyprinus carpio), turbot (Scophthalmus maximus) and Atlantic cod (Gadus morhua). The degree of cross-reactivity differs between species and with the methods employed. The monoclonal antibody works well in both ELISA and western blot for a number of species. The antibody also works well in immunohistochemistry.
Citations
Publication ()
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