Clostridium Perfringens NetB ELISA Kit

Name: Clostridium Perfringens NetB ELISA Kit
SKU: DEIASL175
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma

Species Reactivity

Chicken

Intended Use

This kit is intended for detection of Clostridium perfringens Necrotic Enteritis Toxin B (NetB) concentration in chicken serum, plasma and related liquid samples.

Storage

This kit should be stored at a temperature of 2-8 ° C. The shlef life is 6 months.

Detection Range

3ng/L-140ng/L

General Description

Clostridium perfringens is a Gram-positive, rod-shaped, anaerobic, spore-forming bacterium of the genus Clostridium. C. perfringens is ubiquitous in nature and can be found as a normal component of decaying vegetation, marine sediment, the intestinal tract of humans and other vertebrates, insects, and soil.C. perfringens is commonly encountered in infections as a benign component of the normal flora. In this case, its role in disease is minor. Infections due to C.perfringens show evidence of tissue necrosis, bacteremia, emphysematous cholecystitis, and gas gangrene, which is also known as clostridial myonecrosis.

Citations

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Use of Selected Plant Extracts in Controlling and Neutralizing Toxins and Sporozoites Associated with Necrotic Enteritis and Coccidiosis

Khan MM, Lillehoj HS, Lee Y, Adetunji AO, Omaliko PC, Kang HW, Fasina YO.

Applied Sciences2024 AprRead Article

Applications: ELISA
Reactive species: Clostridium Perfringens
"Abstract: Due to increasing concerns about the contamination of animal food products with antibiotic-resistant bacteria and their byproducts, phytogenic feed additives in animal diets have been explored as antibiotic alternatives. In this study, we investigated the effect of ginger root extract (GRE), green tea extract (GTEC caffeinated and GTED decaffeinated), and onion peel combined (OPEC) on the activity of C. perfringens toxin genes and Eimeria tenella sporozoites. To this end, two Clostridium perfringens strains, CP19 and CP240 (Rollins Diagnostic Lab, Raleigh, NC, USA), were cultured (three replicates per treatment) as follows: without additives (Control), with Bacitracin Methylene Disalicylate (BMD), with GRE, with GTEC, with GTED, and, finally, with OPEC for 0, 2, 4, 6, 8, and 24 h. RNA was extracted to determine the expression of tpeL, alpha toxin (α-toxin), and NetB and we measured the protein concentration of NetB-positive C. perfringens toxin. Also, we evaluated the cytotoxic effect of green tea and ginger extracts on E. tenella sporozoites. Results show that phytogenic extracts, GRE, GTEC, and GTED, significantly reduced (p < 0.05) the level of expression of α-toxin gene compared to control; however, BMD treatment showed much less effect. Furthermore, NetB and tpeL encoding gene expression was significantly (p < 0.05) reduced by GRE and GTED, as well as BMD treatment, compared to the control. In contrast, GTEC treatment did not change the expression levels of these genes and was similar to control."
Article snippet: Frozen clostridium culture samples with or without phytogenic extract or BMD were thawed on ice and subjected to an enzyme-linked immunosorbent assay (ELISA) procedure for the determination of antibody levels to recombinant NetB C. Perfringens protein according to the manufacturer’s instruction (Creative Diagnostics, Shirley, NY, USA).