Ciprofloxacin and Enrofloxacin ELISA Kit

Sample

tissue (chicken and duck meat), aquatic products (fish and shrimp meat), and eggs (chicken eggs)

Detection Method

cELISA

Intended Use

This product can be used for the qualitative and quantitative detection of ciprofloxacin and enrofloxacin residues in tissue (chicken and duck meat), aquatic products (fish and shrimp meat), and eggs (chicken eggs).

Contents of Kit

1. 96-well microplate × 1 (pre-coated with conjugated antigen)
2. Standard solutions × 5 vials (1 mL/vial): 0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb (1 ppb = 1 μg/L = 1 μg/kg)
3. High-concentration standard (1 mL/vial): 10 ppm
4. Enzyme conjugate concentrate (1 mL, red cap)
5. Enzyme conjugate diluent (10 mL, green cap)
6. Substrate A (7 mL, white cap)
7. Substrate B (7 mL, red cap)
8. Stop solution (7 mL, yellow cap)
9. 20× Concentrated wash buffer (40 mL, transparent cap)
10. 2× Concentrated reconstitution solution (50 mL, blue cap)

Storage

Storage: 2–8°C
Shelf life: 12 months

Performance Characteristics

Accuracy: Tissues, aquatic products, and eggs: 100% ± 30%

Precision

Intra-assay and inter-assay coefficients of variation are both less than 10%.

Detection Limit

Detection Limit for samples:
Tissues, aquatic products, and eggs (Method 1): 0.2 ppb
Tissue samples (Method 2): 10 ppb

Sensitivity

0.05 ppb

General Description

Fluoroquinolones (QNS) are a highly important class of broad-spectrum antibiotics that have developed rapidly over the past 20 years. They inhibit bacterial DNA gyrase and are characterized by a broad antibacterial spectrum, high efficacy, low toxicity, and strong tissue penetration. They have become one of the most important anti-infective drugs in veterinary clinical practice and aquaculture, widely used for treatment, prevention, and growth promotion. Their resistance and potential carcinogenicity have raised widespread concern.
In tissues, the marker residues for enrofloxacin are enrofloxacin and ciprofloxacin. The highest residue concentrations are found in liver and kidney tissues, followed by muscle and skin with adhering fat. The metabolite ciprofloxacin (CIF) of enrofloxacin still possesses biological activity.
This kit is a new generation of drug residue detection product developed using ELISA technology. Compared with instrumental analysis techniques such as high-performance liquid chromatography (HPLC), it offers the advantages of speed, simplicity, accuracy, and high sensitivity. The total operation time is only 45 minutes, minimizing operational errors and workload.

General Notes

Fluoroquinolones (QNS) are a highly important class of broad-spectrum antibiotics that have developed rapidly over the past 20 years. They inhibit bacterial DNA gyrase and are characterized by a broad antibacterial spectrum, high efficacy, low toxicity, and strong tissue penetration. They have become one of the most important anti-infective drugs in veterinary clinical practice and aquaculture, widely used for treatment, prevention, and growth promotion. Their resistance and potential carcinogenicity have raised widespread concern.
In tissues, the marker residues for enrofloxacin are enrofloxacin and ciprofloxacin. The highest residue concentrations are found in liver and kidney tissues, followed by muscle and skin with adhering fat. The metabolite ciprofloxacin (CIF) of enrofloxacin still possesses biological activity.
This kit is a new generation of drug residue detection product developed using ELISA technology. Compared with instrumental analysis techniques such as high-performance liquid chromatography (HPLC), it offers the advantages of speed, simplicity, accuracy, and high sensitivity. The total operation time is only 45 minutes, minimizing operational errors and workload.