Product Overview
A DNA sequence encoding the chinese hamster PLA2G15 (G3HKV9-1) (Val34-Pro412) was expressed with a polyhistidine tag at the C-terminus.
Molecular Weight
The recombinant chinese hamster PLA2G15 consists of 390 amino acids and predicts a molecular mass of 45 kDa. It migrates as an approximately 53.5 kDa band in SDS-PAGE under reducing conditions.
Alternative Names
PLA2G15; phospholipase A2, group XV; group XV phospholipase A2; GXVPLA2; LLPL
Purity
> 95 % as determined by SDS-PAGE.
Buffer
Lyophilized from sterile PBS, pH 7.4.
Storage
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Introduction
Lysosomal phospholipase A2 (PLA2G15) is a ubiquitous enzyme uniquely characterized by a subcellular localization to the lysosome and late endosome. PLA2G15 has an acidic pH optimum, is calcium independent, and acts as a transacylase in the presence of N-acetyl-sphingosine as an acceptor.
Keywords
PLA2G15; phospholipase A2, group XV; group XV phospholipase A2; GXVPLA2; LLPL
Citations
Publication ()
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