Cas9 ELISA Kit

Sample

Cell lysates, viral vectors, and gene-editing reagents.

Species Reactivity

N/A

Detection Method

sELISA

Intended Use

The Cas9 ELISA Kit provides a rapid and quantitative solution for detecting Streptococcus pyogenes Cas9 (SpCas9) protein in cell lysates, viral vectors, and gene-editing reagents.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Cas9 Capture Plate	1 plate	2-8°C
2	Cas9 Standard Stock	1 vial (50 μL)	2-8°C
3	Biotin Anti-Cas9 Antibody	1 bottle (12 mL)	2-8°C
4	Streptavidin-HRP	1 bottle (12 mL)	2-8°C
5	Sample Dilution Buffer	1 bottle (60 mL)	2-8°C
6	20× Wash Solution	1 bottle (60 mL)	2-8°C
7	TMB Solution	1 bottle (12 mL)	2-8°C
8	Stop Solution	1 bottle (6 mL)	2-8°C
9	Plate Sealer	2 pieces	2-8°C

Storage

The unopened kit is stable for at least 12 months from the date of manufacture at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.

Performance Characteristics

Versatile Detection
This kit is designed for the detection of a wide range of Cas9 proteins, including wild-type SpCas9, high-fidelity mutants (e.g., eSpCas9), and variants featuring nuclear localization signals (NLS) at the N-terminus, C-terminus, or both. Several commercially available Cas9 constructs have been tested, and the typical assay working ranges are presented in Table and Figure below. While not all Cas9 variants have been individually validated, the assay demonstrates broad compatibility and is likely to detect many commonly used formats. Users are encouraged to follow the standard protocol to assess compatibility with their specific Cas9 variant or construct.
Table. Typical assay working ranges for different Cas9 variants

Precision

Intra-assay and inter-assay precision were measured in 3 different concentrations of Cas9 samples, using 3 batches of kits.
Table. Intra-assay and inter-assay precision of the kit

Detection Range

The typical detection range of this kit for Cas9 is from 0.125 to 8 ng/mL according to the evaluation method recommended in ICH Q2(R2)^[1]

Detection Limit

Detection Capability
According to the evaluation method recommended in ICH Q2 (R2) ^[1] and ICH-M10 ^[2], the LLOQ (Lower Limit of Quantification) of this kit is 0.125 ng/mL, and the ULOQ (Upper Limit of Quantification) is 8 ng/mL.
Note: Lower Limit of Quantification (LLOQ) is the lowest amount of an analyte in a sample that can be used for quantification. The upper Limit of Quantification (ULOQ) is the highest amount of an analyte in a sample that can still be used for quantification. 0.125 ng/mL is the lowest quantifiable concentration in our standard curve. Customers are required to verify the appropriate LLOQ for their samples.

General Description

CRISPR-Cas9 is a revolutionary genome-editing system that enables precise DNA modification through RNA-guided double-strand breaks. As the molecular scissors in this system, the Cas9 endonuclease allows targeted gene deletion, insertion, or replacement across diverse biological models.
The Cas9 ELISA Kit provides a rapid and quantitative solution for detecting Streptococcus pyogenes Cas9 (SpCas9) protein in cell lysates, viral vectors, and gene-editing reagents. This measurement is critical for optimizing CRISPR experimental workflows and ensuring quality control in therapeutic development.
This ELISA kit provides broad compatibility with commonly used Cas9 variants, including high-specificity mutants and constructs with single or double nuclear localization signals.
In addition to detecting wild-type Cas9, the kit is validated for multiple commonly used Cas9 constructs with different NLS configurations and enhanced specificity mutations, making it highly versatile for various experimental and therapeutic contexts.