Sample
serum, plasma, cell culture supernatants, tissue homogenate
Species Reactivity
Canine
Detection Method
Sandwich ELISA
Intended Use
For quantitative detection of Canine Histon H3 in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
31.2-2000 pg/mL
Citations
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Kase, S; Yoshida, K; et al. Phosphorylation of p27(KIP1) in the mitotic cells of the corneal epithelium. CURRENT EYE RESEARCH 31:307-312(2006).
Kase, S; Yoshida, K; et al. Phosphorylation of p27(KIP1) in the developing retina and retinoblastoma. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 16:257-262(2005).
COA
Certificate of Analysis
