Native Clostridium difficile Toxoid A

Nature

Native

Tag/Conjugate

Unconjugated

Purity

>95% pure by SDS-PAGE

Format

Lyophilised

Buffer

Contents when reconstituted in 250μl sterile distilled water will contain: Toxoid A at a concentration of 0.4mg/ml in 0.05M Hepes, 0.15M NaCl and 5% sucrose

Preservative

None

Storage

Lyophilised vial: +2°C to +8°C; Reconstituted liquid: +2°C to +8°C. Reconstituted liquid can be frozen at -80°C to -20°C to extend stability to l year; however every freeze/thaw cycle will cause increased aggregation. Avoid multiple freeze/thaw cycles and multiple temperature fluctuations. Mixing: Swirl or pipette gently to mix. Vortexing promotes aggregation.

Stability

Lyophilised vial: 1 year after date of receipt; Reconstituted liquid: 1 month

Antigen Description

Native toxin A purified from C. difficile strain VPI10463 (toxinotype 0), converted to inactive toxoid fonn. Inactivated using formaldehyde. No cell rounding in the sensitive verocytotoxicity assay at 2μg/ml compared to active toxin lethal dose of 0.2ng/ml. The Clostridium difficile Toxoid A is a highly purified, non-toxic preparation derived from C. difficile toxin A. It has been developed either for use as an immunogen to raise antibodies or for use in clinical diagnostic assays. Formaldehyde is use to convert the toxin into Toxoid A and quality control data illustrates that this treatment has no significant effect on the antigenicity of the resultant preparation. The product is presented in a choice of pack sizes and is lyophilised for ease of use.

Keywords

Clostridium difficile Toxoid A; C. difficile Toxoid A; Clostridium difficile Toxin A; C. difficile Toxin A; Toxin A; TcdA; The large clostridial cytotoxins; LCT