Tag/Conjugate
Unconjugated
Molecular Weight
The molecular weight of the protein is approximately 50.600 kDa.
Alternative Names
Clostridium botulinum Neurotoxin Type D
Purity
The protein appears to be greater than 90% pure based on densitometry.
Format
Lyophilized power, sealed under vacuum
Storage
Store lyophilized vials at 4°C. Once dissolved, aliquot and store at -20°C. Refrain from multiple freeze/thaw cycles.
Reconstitution
When reconstituted with 33 μl of sterile distilled water, each vial contains 10 ug of light chain in 20 mM HEPES, pH 7.4 + 1.25% lactose. The pH 7.4 HEPES buffer was obtained by titrating the free acid form of HEPES with the potassium salt form of HEPES. This minimizes the sodium and chloride concentrations present with the toxin. In order to ensure stability during storage of the protein 0.05% TWEEN-20 or 1 mg/ml BSA must be included in your reconstitution buffer.
Antigen Description
The light chain D fragment contains amino acids 1-436 of the full length botulinum neurotoxin type D. It also contains eight residual amino acids from the affinity tag at the Nterminus to give a total length of 444 amino acids.
Citations
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