Boldenone ELISA Kit

Sample

Milk, fish, shrimp, meat ,plasma and urine

Detection Method

cELISA

Intended Use

Boldenone ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of Boldenone in milk, fish, shrimp, meat ,plasma and urine.

Contents of Kit

1. Boldenone-coated Microtiter Plate, 1 × 96-well plate (8 wells × 12 strips), 2-8°C
2. Boldenone Standards: 2-8°C
Negative control (white cap tube), 1mL
0.1ng/mL (yellow cap tube), 1mL
0.3 ng/mL (orange cap tube), 1mL
0.9 ng/mL (pink cap tube), 1mL
2.7 ng/mL (purple cap tube), 1mL
8.1 ng/mL (blue cap tube), 1mL
100 ng/mL (spiking, optional, red cap tube), 1mL
3. Boldenone Antibody #1, 6 mL, 2-8°C *
4. HRP-Conjugated Antibody #2, 6 mL, 2-8°C *
5. 5× Milk Sample Dilution, 25 mL, 2-8°C
6. 10× Sample Extraction Buffer, 30 mL, 2-8°C
7. 20× Wash Solution, 30 mL, 2-8°C
8. Stop Buffer, 12 mL, 2-8°C
9. TMB Substrate, 12 mL, 2-8°C
10. Clean up mix (optional), 30mL, 2-8°C
* If you are not planning to use the kit for over 3 months, store Antibody #1 and HRP-Conjugated Antibody #2 at -20°C or in a freezer.

Storage

Boldenone ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C*. The shelf life is 12 months when the kit is properly stored.
* If you are not planning to use the kit for over 3 months, store Antibody #1 and HRP-Conjugated Antibody #2 at -20°C or in a freezer.

Performance Characteristics

The unique features of the kit are:
Rapid (10-40min), high recovery (>80%), and cost-effective extraction methods .
High sensitivity (0.1 ng/g or ppb) and low detection limit (0.1ng/g or ppb) .
A quick ELISA assay (less than 5hours regardless of number of samples).
High reproducibility.

Sensitivity

Milk: 1.0(ng/g or ppb)
Shrimp/Fish/Meat: 0.1(ng/g or ppb)
Plasma/Serum: 0.05(ng/g or ppb)
Urine: 0.5(ng/g or ppb)