Anti-SHMT2 Polyclonal Antibody (CABT-L2159) Knockout Validated

Rabbit Anti-Human SHMT2 (Asn30~His504) Polyclonal Antibody for WB

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human, Pig
Immunogen
Recombinant fragment corresponding to human SHMT2 (Asn30~His504)
Conjugate
Unconjugated

Applications


Application Notes
Recommended dilution: WB: 0.5-2 ug/ml; 1:200-800
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Knockout Varification: Lane 1: Wild-type 293T cell lysate. Lane 2: SHMT2 knockout 293T cell lysate. Predicted MW: 54kd, Observed MW: 55kd
Figure. Western Blot. Sample. Lane1: Human Lung Tissue; Lane2: Human 293T Cells; Lane3: Human Hela Cells; Lane4: Porcine Pancreas Tissue.

Target


Alternative Names
Serine methylase; Glycine hydroxymethyltransferase
Entrez Gene ID
UniProt ID

Product Background


Pathway
Biosynthesis of amino acids; C1-unit interconversion, eukaryotes; Carbon metabolism; Cyanoamino acid metabolism; Glycine Metabolism; Glycine, serine and threonine metabolism; Glyoxylate and dicarboxylate metabolism; Metabolic pathways;

Citations


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References


GUAN, YH; RAWSTHORNE, S; et al. CLONING AND CHARACTERIZATION OF A DIHYDROLIPOAMIDE ACETYLTRANSFERASE (E2) SUBUNIT OF THE PYRUVATE-DEHYDROGENASE COMPLEX FROM ARABIDOPSIS-THALIANA. JOURNAL OF BIOLOGICAL CHEMISTRY 270:5412-5417(1995).
Lin, HB; Falchetto, R; et al. Mimosine targets serine hydroxymethyltransferase. JOURNAL OF BIOLOGICAL CHEMISTRY 271:2548-2556(1996).

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