Anti-VF Polyclonal Antibody (CABT-L2105) Knockout Validated

Rabbit Anti-Human Visfatin (Met1~His491) Polyclonal Antibody for WB

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human, Pig
Immunogen
Recombinant fragment corresponding to human NAMPT (Met1~His491)
Conjugate
Unconjugated

Applications


Application Notes
Recommended dilution: WB: 0.2-2 ug/ml; 1:250-2500
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Knockout Varification: Lane 1: Wild-type 293T cell lysate. Lane 2: VF knockout 293T cell lysate. Predicted MW: 55kd, Observed MW: 55kd
Western Blot. Sample. Human 293T cell lysate.
Western Blot. Sample. Porcine Heart lysate.
Western Blot. Sample. Porcine Liver lysate.
Western Blot. Sample. Porcine Stomach lysate.

Target


Alternative Names
Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase
UniProt ID

Product Background


Pathway
Adipogenesis; BMAL1:CLOCK,NPAS2 activates circadian gene expression; Circadian Clock; Defective AMN causes hereditary megaloblastic anemia 1; Defective BTD causes biotidinase deficiency; Defective CD320 causes methylmalonic aciduria; Defective CUBN causes hereditary megaloblastic anemia 1; Defective GIF causes intrinsic factor deficiency;

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Jacques, C; Holzenberger, M; et al. Proinflammatory Actions of Visfatin/Nicotinamide Phosphoribosyltransferase (Nampt) Involve Regulation of Insulin Signaling Pathway and Nampt Enzymatic Activity. JOURNAL OF BIOLOGICAL CHEMISTRY 287:15100-15108(2012).
Montecucco, F; Cea, M; et al. Nicotinamide Phosphoribosyltransferase as a Target in Inflammation-Related Disorders. CURRENT TOPICS IN MEDICINAL CHEMISTRY 13:2930-2938(2013).

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