Anti-Zaire Ebola Virus VP35 Protein monoclonal antibody (CABT-B292)

Mouse Anti-Zaire Ebola Virus VP35 Protein (C-Terminal) monoclonal antibody for WB, IP, IF

Specifications


Host Species
Mouse
Antibody Isotype
IgG2a
Clone
7D6
Species Reactivity
Virus
Immunogen
DNA immunization followed by recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
WB: 1:1000
IP: 1:500
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


EBOV VP35 S187A impairs VP35 phosphorylation in HepG2 cells. HepG2 cells were transfected with plasmids pCAGGS-VP35, pCAGGS-VP35 S187A, pCAGGS-VP35
S205A/T206A/S208A, pCAGGS-VP35 S317A and pCAGGS-VP35 S187A/S205A/T206A/
S208A/S317A as indicated, and the cell lysates were subjected to anti-VP35 immunoprecipitation. Immunoprecipitation was detected by immunoblotting with phosphoserine, VP35 and b-actin antibodies as indicated.

Product Background


Antigen Description
Ebola virus (EBOV), or sometimes referred to as Zaire ebolavirus is one of the five identified Ebola virus species. Zaire ebolavirus is a virological taxon that comes from the genus, Ebolvarius, family Filoviridae and the order Mononegavirales. Ebola, often fatal has been shown to cause hemorrhagic fever in humans and other mammals including monkeys and chimpanzees. Polymerase cofactor VP35 acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. It prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3). This blockage is produced through the interaction with and inhibition of host IKBKE and TBK1. VP35 also inhibits the antiviral effect mediated by the host interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR.

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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Zhu, Lin, et al. "Ebola virus replication is regulated by the phosphorylation of viral protein VP35." Biochemical and biophysical research communications (2019).

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