Human YY1 peptide

The present study was designed to estimate the relative gene expression of major genes responsible for beta-casein and its transcription regulation during early lactation cycle in Surti and Jaffarabadi buffaloes. Ten buffaloes of each breed maintained at Livestock Research Station, Navsari and Cattle Breeding Farm, Junagadh, respectively were selected for the study. The RNA was extracted from noninvasively isolated primary buffalo mammary epithelial cells (pBMEC) obtained using antibody mediated magnetic separation method from milk samples collected at day 15 and 60 postpartum (pp). Primers used for amplification of beta-casein gene (CSN2) and its transcription regulatory genes were bovine specific. The mean relative expressions of C/EBP beta, RUNX2 and STAT5A genes were significantly higher in Jaffarabadi buffaloes as compared to Surti buffaloes at day 15 pp. However, between breed differences in the mean relative expressions of C/EBP beta, RUNX2 and STAT5A genes were not observed at 60 days pp. The relative expressions of CSN2 and YY1 genes did not differ significantly within or between breeds. The mean relative expression of KRT8 gene was almost comparable among all the groups under present study. Thus, only beta casein transcription regulatory genes C/EBP beta, RUNX2 and STAT5A amongst all studied genes showed significant between breed variability at day 15 pp, however this variability too got subsided as lactation stage advanced to day 60 pp.

RING1 and YY1 binding protein (Rybp) genes inhibit myogenesis in mice, but there are no reports on the effects of these genes in cattle. The aim of this study is to investigate the roles of the Rybp gene on bovine skeletal muscle development and myoblast differentiation. In the present study, the Rybp gene was overexpressed in bovine myoblasts via adenovirus. RNA-seq was performed to screen differentially expressed genes (DEGs). The results showed that overexpressing the Rybp gene inhibits the formation of myotubes. The morphological differences in myoblasts began on the second day and were very significant 6 days after adenovirus induction. A total of 1311 (707 upregulated and 604 downregulated) DEGs were screened using RNA-seq between myoblasts with added negative control adenoviruses (AD-NC) and Rybp adenoviruses (AD-Rybp) after 6 days of induction. Gene ontology (GO) and KEGG analysis revealed that the downregulated DEGs were mainly involved in biological functions related to muscle, and, of the 32 pathways, those associated with muscle development were significantly enriched for the identified DEGs. This study can not only provide a theoretical basis for the regulation of skeletal muscle development in cattle by exploring the roles of the Rybp gene in myoblast differentiation, but it can also lay a theoretical foundation for molecular breeding of beef cattle.