Vitamin D3 ELISA Kit

Name: Vitamin D3 ELISA Kit
SKU: DEIA4442A
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

dairy samples

Detection Method

cELISA

Intended Use

The Vitamin D3 ELISA Kit is for the quantitative determination of Vitamin-D3 in dairy samples.

Contents of Kit

1. Vitamin-D3 coated wells: 96 wells with Vitamin-D3 immobilized in the well, in a foil pouch with a dessicant.
2. Anti-Vitamin D3 conjugate with HRP: one vial containing 0.1 mL of concentrated Anti-Vitamin-D3 conjugate with HRP, in a stabilizer solution.
3. Vitamin-D3 Standard: Standards prepared with hexane: 0, 0.125, 0.25, 0.50, 0.75 IU/mL. Content is 0.5 mL per vial.
4. CONTROL 1: Control 0.6 IU/mL, 0.5 mL per vial.
5. CONTROL 2: Control 0.2 IU/mL, 0.5 mL per vial.
6. Reaction Buffer: one vial containing 7 mL of peptide based buffer with thimerosal as preservative.
7. Enzyme substrate: one vial containing 7 mL of TMB solution.
8. Conjugate Diluent: one vial containing 7 mL of carbohydrate based buffer with thimerosal as preservative.
9. H₂SO₄ Stopping solution: one vial containing 7 mL of 0.2 M sulfuric acid.

Storage

Storage: 2 - 8°C.
Stability: refer to expiration date on reagent labels.

Precision

The relative standard deviation for interassay and intrassay was determined to be 8% and 4% respectively.

Sensitivity

The range for this assay under the specified conditions is from 0.125 I.U./mL to 0.75 I.U./mL.

General Description

Vitamin D is a group of fat-soluble secosteroids, the two major physiologically relevant forms of which are vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). Vitamin D without a sub-script refers to either D2 or D3 or both. Vitamin D3 is produced in the skin of vertebrates after exposure to ultraviolet B light from the sun or artificial sources, and occurs naturally in fish and a few other foods. In some countries, staple foods such as milk, flour and margarine are artificially fortified with vitamin D, and it is also available as a supplement in pill form. Light-exposed mushrooms may provide up to 100% of the recommended Daily Value of vitamin D.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

TSLP is differentially regulated by vitamin D3 and cytokines in human skin

IMMUNITY INFLAMMATION AND DISEASE

Authors: Landheer, Janneke; Giovannone, Barbara; Sadekova, Svetlana; Tjabringa, Sandra; Hofstra, Claudia; Dechering, Koen; Bruijnzeel-Koomen, Carla; Chang, Charlie; Ying, Yu; Malefyt, Rene De Waal; Hijnen, DirkJan; Knol, Edward

Abstract

Thymic stromal lymphopoietin (TSLP) plays an important role in allergic diseases and is highly expressed in keratinocytes in human lesional atopic dermatitis (AD) skin. In nonlesional AD skin TSLP expression can be induced by applying house dust mite allergen onto the skin in the atopy patch test. Several studies have demonstrated that the induction of TSLP expression in mouse skin does not only lead to AD-like inflammation of the skin, but also predisposes to severe inflammation of the airways. In mice, TSLP expression can be induced by application of the 1,25-dihydroxyvitamin D3 (VD3) analogue calcipotriol and results in the development of eczema-like lesions. The objective is to investigate the effect of VD3 (calcitriol) or calcipotriol on TSLP expression in normal human skin and skin from AD patients. Using multiple ex vivo experimental setups, the effects of calci(po) triol on TSLP expression by normal human skin, and skin from AD patients were investigated and compared to effects of calcipotriol on mouse and non-human primates (NHP) skin. No induction of TSLP expression (mRNA or protein) was observed in human keratinocytes, normal human skin, nonlesional AD skin, or NHP skin samples after stimulation with calcipotriol or topical application of calcitriol. The biological activity of calci(po) triol in human skin samples was demonstrated by the increased expression of the VD3-responsive Cyp24a1 gene. TSLP expression was induced by cytokines (IL-4, IL-13, and TNF-alpha) in skin samples from all three species. In contrast to the findings in human and NHP, a consistent increase in TSLP expression was confirmed in mouse skin biopsies after stimulation with calcipotriol. VD3 failed to induce expression of TSLP in human or monkey skin in contrast to mouse, implicating careful extrapolation of this often-used mouse model to AD patients.

Vitamin D3 and Beta-carotene Deficiency is Associated with Risk of Esophageal Squamous Cell Carcinoma - Results of a Case-control Study in China

ASIAN PACIFIC JOURNAL OF CANCER PREVENTION

Authors: Huang, Gui-Ling; Yang, Lei; Su, Ming; Wang, Shao-Kang; Yin, Hong; Wang, Jia-Sheng; Sun, Gui-Ju

Abstract

Objective: The aim was to evaluate roles of vitamin D3 (VD3) and beta-carotene (BC) in the development of esophageal squamous cell carcinoma (ESCC) in a high-risk area, Huai'an District, Huai'an City, China. Methods: 100 new ESCC diagnosed cases from 2007 to 2008 and 200 residency- age-, and sex-matched healthy controls were recruited. Data were collected from questionnaires, including a food frequency questionnaire (FFQ) to calculate the BC intake, and reversed phase high-performance liquid chromatography (RP-HPLC) was used to measure the serum concentrations of BC and VD3. Odds ratios (OR) and 95% confidence intervals (CI) were calculated in conditional logistic regression models. Results: The average dietary intake of BC was 3322.9 mu g (2032.4- 5734.3) in the case group and 3626.8 mu g (1961.9-5827.9) in control group per capita per day with no significant difference by Wilcoxon test (p>0.05). However, the levels of VD3 and BC in the case group were significantly lower than in the control group (p<0.05). The OR values of the highest quartile and the lowest quartile of VD3 and BC in serum samples were both 0.13. Conclusion: Our results add to the evidence that high circulating levels of VD3 and BC are associated with a reduced risk of ESCC in this Chinese population.