Magic™ Anti-VASP (phospho S239) polyclonal antibody

The objective of this study was to determine whether AMPK is activated by 2-arachidonoylglycerol (2-AG) and participates to the cytoskeleton control in human platelets. We found that 2-AG stimulates the AMPK activation through a Ca2+/Calmodulin-dependent pathway as the specific inhibition of the CaMKK by STO-609 inhibits the AMPK phosphorylation/activation. Moreover, the CaMKK/AMPK pathway activated by 2-AG is involved in the phosphorylation of cofilin, vasodilator stimulated phosphoprotein (VASP), and myosin light chain (MLCs). These proteins participate to actin cytoskeletal remodelling during aggregation. We found that the phosphorylation/activation inhibition of these proteins is associated with a significant reduction in actin polymerization, aggregation, ATP, and -granule secretion. Finally, AMPK activation, Cofilin, VASP, and MLCs phosphorylation are significantly reduced by SR141716, the specific inhibitor of type 1 cannabinoid (CB1) receptor, suggesting that the CB1 receptor is involved in the 2-AG effect. In conclusion, we have shown that the CaMKK/AMPK pathway is activated by 2-AG in human platelets and controls the phosphorylation of key proteins involved in actin polymerization and aggregation.

In this work we present results of ab-initio investigation atomic structures and magnetic properties of Cobalt-based full-Heusler compounds with composition Co(2)YZ by using VASP software package. The lattice parameters of Co2FeAl(CFA), Co2FeSi(CFS), Co2FeAl0.5Si0.5(CFAS) Heusler alloys depending on the parameters of convergence are calculated. Investigation of CFA and CFS alloys in B2-type and L2(1)-type structures with determination of their energy efficiency of the formation are carried out. The values of the magnetic moment of CFAS atoms in the bulk and in CFAS/Ag(100)/CFAS multilayer structure taking into account the relaxation effects are determined.