Product Overview
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The USP48 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Alternative Names
USP48; ubiquitin specific peptidase 48; ubiquitin specific protease 31; ubiquitin specific protease 48; USP31; ubiquitin carboxyl-terminal hydrolase 48; FLJ11328; FLJ20103; FLJ23054; FLJ23277; MGC14879; ubiquitin thioesterase 48; deubiquitinating enzyme 48; ubiquitin thiolesterase 48; ubiquitin specific protease 31; ubiquitin specific protease 48; ubiquitin-specific-processing protease 48; USP31; RAP1GA1; MGC132556; DKFZp762M1713;
Application Notes
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Dilution
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
Concentration
The protein concentration was determined with BCA assay.
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
Citations
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Zheng, JZ; Bizzozero, OA; et al. Decreased activity of the 20S proteasome in the brain white matter and gray matter of patients with multiple sclerosis. JOURNAL OF NEUROCHEMISTRY 117:143-153(2011).
Armando, I; Villar, VM; et al. Dopamine D3 receptor inhibits the ubiquitin-specific peptidase 48 to promote NHE3 degradation. FASEB JOURNAL 28:1422-1434(2014).
COA
Certificate of Analysis
