General Notes
This product (Catalog No. CABT-BL3747) is a rabbit-derived polyclonal antibody raised against human USMG5, targeting the amino acid region 1–58 of the protein. This antibody exhibits cross-reactivity with Human USMG5 from mouse and rat. It has been affinity purified by Protein A. ATP5MK (previously known as USMG5 or ATP5MD) is a human gene located on chromosome 10q24.33 that encodes a small 58-amino acid protein, known as ATP synthase membrane subunit k, up-regulated during skeletal muscle growth protein 5 (USMG5), or ATP synthase membrane subunit DAPIT (ATP5MD), which serves as a subunit of both the mitochondrial ATP synthase complex V and the lysosomal V-ATPase. The protein features a central transmembrane domain, a dilysine motif for organelle targeting, and a cytoplasmic C terminus, and it is phylogenetically conserved across vertebrates and invertebrates. Functionally, ATP5MK associates stoichiometrically with the F0 portion of mitochondrial ATP synthase, acting as a chaperone to promote its assembly and dimerization into supercomplexes that maintain cristae structure and optimize proton flow for efficient ATP production from ADP and inorganic phosphate. Knockdown or knockout of ATP5MK reduces steady-state levels of ATP synthase subunits, impairs ATP synthesis across various respiratory substrates, and compromises cell viability, particularly under glucose deprivation, without affecting the assembly or activity of other respiratory chain complexes (I–IV). Expression of ATP5MK is highest in insulin-sensitive tissues such as skeletal muscle (preferentially oxidative fibers), epithelial cells, and neurons, and it is downregulated in models of diabetes, suggesting a role in metabolic regulation.
CABT-BL3747 is suitable for use in Western blot (WB) (1:500–1:1,000), IHC (1:50–1:500) applications.
CABT-BL3747 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
COA
Publication 
Loading ......
