Tyrosine Colorimetric Assay Kit

Sample

Serum, Plasma, Urine, Cell lysates, Tissue lysates

Detection Method

colorimetric assay

Intended Use

The Tyrosine Assay Kit is a simple colorimetric assay that measures the total amount of free tyrosine present in foods or biological samples in a 96-well microtiter plate format.

Contents of Kit

1. Tyrosine Standard: One 100 μL vial at 100 mM.
2. 10X Assay Buffer: One 25 mL bottle.
3. 10X Enzyme: One 500 μL vial.

Storage

Upon receipt, store the Tyrosine Standard and 10X Enzyme at -20°C. Store the 10× Assay Buffer at room temperature.

General Description

Tyrosine (also known as 4-hydroxyphenylalanine) is one of the 20 amino acids that serve as building blocks to form peptides and proteins. As a part of proteins, tyrosine plays a role in signal transduction since phosphate groups are transferred to the hydroxyl group of the phenolic side chain of tyrosine by receptor tyrosine kinases, resulting in a change in activity of the target phosphorylated protein. In addition, a tyrosine side chain contributes to the process of photosynthesis. In photosystem II of chloroplasts, tyrosine residue 161 of P680 acts as an electron donor to reduce oxidized chlorophyll. In plants and lower microorganisms, tyrosine is synthesized from the precursor prephenate (the latter also an intermediate to synthesis of shikimate). Prephenate is decarboxylated to create p-hydroxyphenylpyruvate, which is then transaminated to yield tyrosine and α-ketoglutarate. In animals, tyrosine is created from dietary sources of the essential amino acid phenylalanine. Phenylalanine is converted to tyrosine by the enzyme phenylalanine hydroxylase.
Tyrosine is a neurotransmitter precursor and increases the levels of plasma neurotransmitters such as norepinephrine and dopamine. A number of studies have found tyrosine to reduce stress under conditions of sleep deprivation, prolonged work, cold, or fatigue, and includes reductions in stress hormone levels, and stress-induced weight loss. In addition, improvements in cognitive and physical performance have been observed in humans.

Standard Curve

The following figures demonstrate typical Tyrosine Assay Kit results. One should use the data below for reference only. This data should not be used to interpret or calculate actual sample results.